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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 2413-2422
DOI: https://doi.org/10.20546/ijcmas.2018.704.277


In vitro Studies in Litchi chinensis - Effect of Explant and Medium
Kumari Anjani and Harsh Kumar
Department of Agricultural Biotechnology and Molecular Biology, Dr. Rajendra Prasad Central Agricultural University, Pusa-848125, Bihar, India
*Corresponding author
Abstract:

Litchi is a very important fruit of the Indian subcontinent and has a great demand in local and international market. But it is a very troublesome plant to cultivate and the current production rate cannot meet the growing demands of litchi. The in vitro techniques can be used to rapidly increase production. The present study was carried out to develop a rapid micropropagation protocol for litchi by studying the effect of medium and the explant used. The apical bud was found to be the most suitable explant among the four explants used. Out of the nine medium used for the study, MS 1, MS 2 MS 7 and MS 9 were the most effective medium for callogenesis in apical bud. The frequency of callogenesis was more on medium containing 2,4-D in higher concentration and low kinetin and also in medium with higher concentration of kinetin and low 2,4-D.


Keywords: Litchi, Kinetin, Callogenesis
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How to cite this article:

Kumari Anjani and Harsh Kumar. 2018. In vitro Studies in Litchi chinensis - Effect of Explant and Medium.Int.J.Curr.Microbiol.App.Sci. 7(4): 2413-2422. doi: https://doi.org/10.20546/ijcmas.2018.704.277