Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 2329-2340
DOI: https://doi.org/10.20546/ijcmas.2018.704.267


Antibacterial Effects of Eisenia fetida, Earthworm Extract against Pathogenic Bacteria Isolated from Cyprinus carpio
Nitish Bansal1*, Rajender Kumar Gupta1 and Vikas Nehra2
1Department of Zoology and Aquaculture, Chaudhary Charan Singh Haryana Agricultural University, Hisar (Haryana) - 125004, India
2Department of Veterinary Pathology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar (Haryana) - 125004, India
*Corresponding author
Abstract:

In the present investigations, whole body extract of earthworm, Eisenia fetida was tested for antibacterial activity against fish bacterial pathogens. The bacteria were isolated from different tissues of infected common carp, Cyprinus carpio and identified by gram staining method. A total of eight pathogenic bacteria were isolated. Antimicrobial activity of the extract was studied under in vitro conditions by well diffusion assay. The extract of Eisenia fetida showed antibacterial activity against all the pathogenic bacteria and the maximum zone of inhibition was observed against A. hydrophila. Histological investigations had revealed that feeding the fish on a diet prepared by incorporating earthworm extract resulted in an increase in intestinal folds while injecting the fish with A. hydrophila resulted in degeneration and desquamatation of intestinal epithelium feeding on supplementary diet. When a healthy carp was injected with pathogenic bacterium and also fed on a diet prepared in earthworm extract, it resulted in a significant (P<0.05) increase in intestinal folds, intestinal microvilli, increase in concentration of melano-macrophage centre and also haemopoietic tissue in the kidneys. From these studies, it was concluded that the earthworm extract possesses very strong antibacterial properties which could be employed in treating bacterial infections in fishes, leading to the availability of antibiotic free fishes to the consumers.


Keywords: Bacteria, Common carp, Eisenia fetida, Antibacterial activity, Intestine, Kidney
Download this article as Download

How to cite this article:

Nitish Bansal, Rajender Kumar Gupta and Vikas Nehra. 2018. Antibacterial Effects of Eisenia fetida, Earthworm Extract against Pathogenic Bacteria Isolated from Cyprinus carpio.Int.J.Curr.Microbiol.App.Sci. 7(4): 2329-2340. doi: https://doi.org/10.20546/ijcmas.2018.704.267