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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 2000-2020
DOI: https://doi.org/10.20546/ijcmas.2018.704.231


Biochemical and Molecular Characterization of Parents and its Crosses for High Oleic Acid Content in Sunflower (Helianthus annuus L.)
I.S. Tilak1, B. Kisan1, I. Shanker Goud2, Mahantesha B.N. Naika3, Ayyangouda Patil1, Vikas Kulkarni2, Kadirvel Palchamy4 and Praduman Yadav4
1Department of Molecular Biology and Agricultural Biotechnology, College of Agriculture, University of Agricultural Sciences, Raichur-584-104, Karnataka, India
2All India Co-ordinated Research Project on Sunflower, Main Agricultural Research Station, University of Agricultural Sciences, Raichur-584-104, Karnataka, India
3Department of Biotechnology and Crop Improvement, K.R.C. College of Horticulture, Arabhavi-591218, University of Horticultural Sciences, Bagalkot, Karnataka, India
4Indian Institute of Oilseeds Research, Rajendranagar,
Hyderabad -500-030, Telangana, India
*Corresponding author
Abstract:

Globally, Sunflower (Helianthus annuus L.) is one of the most important cultivated oilseed crops. High oleic sunflower oil maintains oil stability also offers a trans-free oil solution for customers. They help in diminishing the cholesterol leading to a reduction in heart diseases. High oleic acid content genotypes have been developed whose oil has higher oxidative stability and better dietary properties than standard genotypes. An investigation was envisaged to evaluate 64 genotypes (seven CMS lines, seven restorer lines, 49 derived crosses and RSFH-1 hybrid as a check) using gas chromatography (GC) and molecular markers to identify high oleic acid associated genotypes. Total, five simple sequence repeats (SSR) markers were chosen for screening high oleic acid content genotypes, out of which only one marker N1-3F showed polymorphism between high and low oleic acid genotypes. Genotypes containing high-oleic acid were found A 1, A 2, A 4 and A 7 line in CMS, R 4, R 5, R 6 and R 7 lines in restorer, and A 1 x R 4, A 1 x R 5, A 2 x R 2, A 2 x R 4, A 2 x R 7, A 4 x R 2, A 4 x R 6 lines in derived crosses and marker N1-3F which has exhibited 749 bp band length and further, results were also confirmed by determining the fatty acid composition. The results of this work allowed to validation of one SSR marker in sunflower genotypes for high oleic acid trait.


Keywords: Helianthus annuus L., High-oleic acid, Gas Chromatography, SSR marker
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How to cite this article:

Tilak, I.S., B. Kisan, I. Shanker Goud, Mahantesha, B.N. Naika, Ayyangouda Patil, Vikas Kulkarni, Kadirvel Palchamy and Praduman Yadav. 2018. Biochemical and Molecular Characterization of Parents and its Crosses for High Oleic Acid Content in Sunflower (Helianthus annuus L.).Int.J.Curr.Microbiol.App.Sci. 7(4): 2000-2020. doi: https://doi.org/10.20546/ijcmas.2018.704.231