Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 881-893
DOI: https://doi.org/10.20546/ijcmas.2018.704.095


Magnetic Nanoparticle Immobilized Cellulase Enzyme for Saccharification of Paddy Straw
Ajay Kumar, Surender Singh* and Lata Nain
Division of Microbiology, ICAR-Indian Agricultural Research Institute, New Delhi-110 012, India
*Corresponding author
Abstract:

The production of the bioethanol from the vast quantity of ligno-cellulosic (LC) biomass using cellulases and yeasts has been suggested as an alternate energy source and offers potential to reduce the burden on fast depleting fossil fuel reservoirs. The major bottlenecks in commercial production of second generation bioethanol are complex feed stocks, high cost of enzymes and non-availability of co-fermenting yeasts. Immobilization of commercial enzyme (Cellic CTec3) on five nanoparticle (Iron oxide, Silicon oxide, Magnesium oxide, Zinc oxide and Silver oxide) were studied by two different methods- physical adsorption and covalent coupling. For covalent coupling method, nanoparticles were activated with aminopropyltriethoxysilane (APTES). The enzyme-nanoparticle formulations were screened in the terms of protein binding and immobilization efficiency. Iron oxide-enzyme formulations were found to perform best with 60 to 80% immobilization efficiency. Besides better thermo-tolerance, the covalently immobilized enzyme showed better catalytic efficiency. The saccharification yields of the free and immobilized enzyme under optimized condition (60 ËšC temperature, 5.0 pH and 6% substrate loading) were compared. The commercial immobilized enzyme showed slightly lower sugar yield (314.77 mg/gds) as compared to the free enzyme (366.6 mg/gds) with 45 % enzyme activity recovery. Bioethanol production from the lignocellulosic biomass can be made economical feasible by using the covalently immobilized magnetic enzyme nanoparticle complex. Besides bioethanol, the prepared enzyme formulation can be used in textile, detergents and food industries.


Keywords: Cellic CTec3, APTES, Immobilization, Saccharification
Download this article as Download

How to cite this article:

Ajay Kumar, Surender Singh and Lata Nain. 2018. Magnetic Nanoparticle Immobilized Cellulase Enzyme for Saccharification of Paddy Straw.Int.J.Curr.Microbiol.App.Sci. 7(4): 881-893. doi: https://doi.org/10.20546/ijcmas.2018.704.095