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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 514-519
DOI: https://doi.org/10.20546/ijcmas.2018.704.060


Standardization of in vitro Mass Multiplication Protocol for Gerbera cv. Partrizia
Shyama Kumari*, Awdhesh Kumar Pal, Subhashish Sarkhel, Paramveer Singh and Randhir Kumar
Bihar Agricultural College, BAU, Sabour-813210, Bhagalpur, Bihar, India
*Corresponding author
Abstract:

An effective protocol for in vitro proliferation and multiplication of Gerbera cv. Partrizia was established during 2015-17. Among the two stages initially used for culture initiation, capitulum explants cultured at immature stage responded better than the mature stage. Best establishment of immature capitulum explants and more number of quality shoots was obtained on modified MS medium supplemented with 10 mg/l BAP and 1 mg/l IAA. Half strength MS medium containing NAA (0.5 mg/l) + IBA (0.5 mg/l) was best for root induction. The regenerated plantlets were efficiently hardened in glass jars filled with vermiculite + agropeat (1:2) moistened with half-strength MS medium salts and covered with polypropylene lids, thereafter plants were successfully transferred to the greenhouse with good survival. This protocol can produce 32,768 to 1,65,880 vigorous, healthy and true to the type plants/year from a single capitulum.


Keywords: Gerbera, Micropropagation, in vitro regeneration, Capitulum, Explant
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How to cite this article:

Shyama Kumari, Awdhesh Kumar Pal, Subhashish Sarkhel, Paramveer Singh and Randhir Kumar. 2018. Standardization of in vitro Mass Multiplication Protocol for Gerbera cv. Partrizia.Int.J.Curr.Microbiol.App.Sci. 7(4): 514-519. doi: https://doi.org/10.20546/ijcmas.2018.704.060