International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:4, April, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 53-61

A Novel Media Optimized for Production of Pullulan in Flask Type Fermentation System
Ranjan Singh1*, Rajeeva Gaur2, Prabhash K. Pandey3, Farrukh Jamal4, Laxmi K. Pandey5, Sangram Singh4, Harish K. Kewat6, Soni Tiwari2, Pritha Biswas1 and Manogya K. Gaur7
1Department of Botany and Microbiology, St. Aloysius College (Autonomous), Jabalpur, M.P., India
2Department of Microbiology (DST-FIST Supported, Centre of Excellence, Govt. of U.P.), Dr. R.M.L. Avadh University, Faizabad, U.P., India
3Genotoxicity Lab, Division of Toxicology, Central Drug Research Institute, Lucknow, U.P., India
4Department of Biochemistry, Dr. R.M.L. Avadh University, Faizabad, U.P., India
5Department of Biotechnology, St. Aloysius College (Autonomous), Jabalpur, M.P., India
6Regional Office, I.G.N.O.U., Jabalpur, M.P., India
7D.G.M. Environment (ETP and Biocompost), Balrampur Distillery, Bhabhanan, Basti, U.P., India
*Corresponding author

Aureobasidium pullulans, popularly known as black yeast, is one of the most widespread saprophytic fungus associated with wide range of terrestrial and aquatic habitats. The fungus has widely been employed in production of an economically important polysaccharide Pullulan. Pullulan is a linear glucan made mainly of maltotriose repeating units. This gives the polysaccharide structural flexibility and enhances solubility. This polysaccharide is of great economic importance with increase applications in food, pharmaceutical, agriculture, blood plasma substitute and chemical industry. Production of pullulan was observed in a novel media consisting of Sugarcane juice (SCJ) and distilled water. It was found that high amount of pullulan (6.4±0.04g/100ml) was obtained at 37oC, pH 5 at a concentration of 50% media in 96h. This is a novel study in which pullulan is grown on media consisting of Sugar Cane Juice (SCJ) and distilled water. Further no synthetic minerals are used in the media for the growth of polysaccharide making it very economical.

Keywords: Aureobasidium pullulans, Pullulan, Sugarcane juice, Production

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How to cite this article:

Ranjan Singh, Rajeeva Gaur, Prabhash K. Pandey, Farrukh Jamal, Laxmi K. Pandey, Harish K. Kewat, Soni Tiwari, Pritha Biswas and Manogya K. Gaur. 2018. A Novel Media Optimized for Production of Pullulan in Flask Type Fermentation System.Int.J.Curr.Microbiol.App.Sci. 7(4): 53-61. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.