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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(7): 778-784
DOI: http://dx.doi.org/10.20546/ijcmas.2016.507.089


A Two Stepwise Molecular Approach for Rapid Separation of Staphylococcus aureus from other Coagulase Negative Staphylococci
Ezeamagu O. Cajethan1*, Fagade E. Obasola2, Smith Stella, I.3 and Ogunjobi A. Adeniyi2
1Department of Biosciences and Biotechnology, Babcock University, Ilishan,
Ogun State, Nigeria 
2Department of Microbiology, University of Ibadan, Oyo State, Nigeria
3Molecular Biology and Biotechnology division, Nigerian Institute of Medical Research,Yaba, Lagos, Nigeria
*Corresponding author
Abstract:

Staphylococcus aureus is the most important cause of nosocomial and community associated infections as well as the most pronounced species in drug resistance amongst Staphylococcus species. There have been reported cases of inconsistency in presumptive coagulase tests using commercial kits and conventional methods. This study therefore reports a simple, rapid, accurate and cost-effective PCR-RFLP-based molecular technique that separates and identifies to species level S. aureus from other coagulase negative staphylococci. Fifty clinical isolates of staphylococci previously isolated from various specimens were investigated for their ability to clot blood plasma. Of the 50 clinical isolates, 6 (12%) and 44 (88%) isolates yielded positive and negative results, respectively, for conventional tube coagulase test. The PCR-RFLP supplemented with species-specific primers classified these isolates into three distinct species: S. epidermidis (86%), S. aureus (12%) and S. xylosus (2%). Furthermore, the technique applied in this study classified the isolates into two RFLP patterns representing S. aureus and other coagulase negative staphylococci which correlated with coagulase test results. Since coagulase test could be sometimes misleading and inconclusive, this method could be substituted in routine laboratory program.


Keywords: S.aureus, coagulase test, PCR-RFLP.
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How to cite this article:

Ezeamagu O. Cajethan, Fagade E. Obasola, Smith Stella, I. and Ogunjobi A. Adeniyi. 2016. A Two Stepwise Molecular Approach for Rapid Separation of  Staphylococcus aureus from other Coagulase Negative Staphylococci.Int.J.Curr.Microbiol.App.Sci. 5(7): 778-784. doi: http://dx.doi.org/10.20546/ijcmas.2016.507.089