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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2018) [Effective from January 1, 2018]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2016: 92.30
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 1803-1808
DOI: https://doi.org/10.20546/ijcmas.2018.703.212


Detection of Entamoeba Species: A Comparative Analysis of Nested-Multiplex PCR and Recombinase Polymerase Amplification
Selvaratthinam Ajay Philips, Kumar Manochitra, Shashiraja Padukone and Subhash Chandra Parija*
Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Puducherry, India
*Corresponding author
Abstract:

Amoebiasis, caused by Entamoeba histolytica, remains a serious public health issue worldwide. For decades, microscopy remains preferred method for detection of the pathogenic species. The recent advent of molecular biology has helped in developing appropriate diagnostic technologies, of which isothermal amplification is considered to be robust and cost-effective compared to PCR that requires expensive instruments and highly skilled personnel. The aim of the present study was to compare the diagnostic ability of nested-multiplex PCR and Recombinase polymerase amplification for differential detection of E. histolytica and E. dispar. The results of this study showed good agreement between the two tests. This suggests that RPA can be used as clinical and epidemiological tool for diagnosis of amoebiasis in resource-limited setups and in endemic regions.


Keywords: Entamoeba histolytica, Species, Nested multiplex PCR
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How to cite this article:

Selvaratthinam Ajay Philips, Kumar Manochitra, Shashiraja Padukone and Subhash Chandra Parija. 2018. Detection of Entamoeba Species: A Comparative Analysis of Nested-Multiplex PCR and Recombinase Polymerase Amplification.Int.J.Curr.Microbiol.App.Sci. 7(3): 1803-1808. doi: https://doi.org/10.20546/ijcmas.2018.703.212