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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(7): 592-598
DOI: http://dx.doi.org/10.20546/ijcmas.2016.507.066


Prevalence of Human Leucocyte Antigen B17 in Spondyloarthritis Patients in a Tertiary Care Center in South India Detected Microlymphocytotoxicity Test
Therese Mary Dhason1*, Euphrasia Latha Jairaj2 and Rajeswari Sankaralingam3
1Senior Assistant Professor of Microbiology (Immunology), Institute of Rheumatology,Madras Medical College, Chennai, India
2Professor of Microbiology (Immunology), Instiute of Rheumatology,Madras Medical College, Chennai, India
3Director and Head, Institute of Rheumatology, Madras Medical College, Chennai, India
*Corresponding author
Abstract:

To detect the prevalence of Human leukocyte antigen (HLA)B17 in Spondyloarthritis (SpA) patients from South India at a Tertiary Care Center by Microlymphocytotoxicity test, a serological method. A case control study was undertaken at a Tertiary Care Center in Chennai to find out the prevalence of HLA-B17 in patients with clinical features of Spondyloarthritis. Blood samples were collected from 75 Spondyloarthritis patients and 85 healthy controls. HLA Class I phenotyping was done by microlymphocytotoxicity test using HLA Terasaki tissue typing trays, procured from One Lambda, California (CA), United States of America (USA). HLA class IB antigen distribution in Spondyloarthritis was B27 in 20(26.66%)patients, B17 in 19(25.33%) and B51 in10(13.33%). The other class IB antigens were detected in low frequency. HLA-A1 was detected in 15(78.94) of the B17 positive Spondyloarthritis patients. Among the controls HLA class IB antigen distribution was B7in12(14.11%)patients B17, B13, B40, B35, in 8 each (9.4%). HLA-A1 was positive in 2(25%) of the B17 positive controls. Prevalence of HLA-B17 in Spondyloarthritis patients (p=0.0104) and prevalence of HLA-A1 (p=0.0248) in B17 positive patients were statistically significant as compared to controls. HLA-B27 was the first common antigen and HLA-B17 was the second common HLA class 1B antigen detected in our SpA patients.  HLA-A1 had an increased prevalence in our patients. Hence we conclude that HLA-B17 and HLA-A1 have a possible role in Spondyloarthritis in our population. The role of these antigens in the pathogenesis of SpA has to be elucidated by large clinical trials.


Keywords: Spondyloarthritis, HLA phenotyping, Microlympho-cytotoxicity test, HLA-B17.
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How to cite this article:

Therese Mary Dhason, Euphrasia Latha Jairaj and Rajeswari Sankaralingam. 2016. Prevalence of Human Leucocyte Antigen B17 in Spondyloarthritis Patients in a Tertiary Care Center in South India Detected Microlymphocytotoxicity Test.Int.J.Curr.Microbiol.App.Sci. 5(7): 592-598. doi: http://dx.doi.org/10.20546/ijcmas.2016.507.066