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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 871-882
DOI: https://doi.org/10.20546/ijcmas.2018.703.102


Isolation and Characterization of Rabbit Gut Transmitted Saccharomyces cerevisiae as a Synbiotic
R. Elanthamil1*, C. Bandeswaran2, P. Tensingh Gnanaraj3 and K. Vijayarani4
1Department of Animal Nutrition, Madras Veterinary College, Chennai, India
2Department of Animal Nutrition, Veterinary College and Research Institute,
Orthanadu, India
3University Research Farm, Madavaram Milk Colony, India
4Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai-600 051, India
*Corresponding author
Abstract:

The use of yeast culture as a dietary supplement has been suggested as a useful tool to stabilize ruminal fermentation. A study was conducted to explore the scope of rabbit gut transmitted (RGT) S. cerevisiae as probiotic and prebiotic (Synbiotic) by in vitro. The rabbit gut transmitted S. cerevisiae was isolated from the faeces of rabbits as follows. Procured Saccharomyces cerevisiae was propagated, freeze dried and supplemented to adult male New Zealand White rabbits (4 x 4) at various doses viz., 0.0 x 108, 1.5 x 108, 3 x 108, 6 x 108 CFU / head / day for a period of 15 days. Significantly (P<0.01) highest Saccharomyces cerevisiae excretion in hard faeces of rabbits was observed at highest supplemental dose of Saccharomyces cerevisiae (3 x 108 CFU / head / day) at 15th day post supplementation. Faecal isolate of Saccharomyces cerevisiae was confirmed for its origin (supplemental Saccharomyces cerevisiae) through morphological, biochemical assay. The faecal isolate was referred to as “RGT Saccharomyces cerevisiae”. Both Saccharomyces cerevisiae and RGT Saccharomyces cerevisiae were assessed for their probiotic and prebiotic characters in seven replications. Probiotic characterization viz., bile tolerance test and pH tolerance test showed that RGT Saccharomyces cerevisiae had significantly (P<0.01) higher bile tolerance (0.3, 0.6 and 0.9 per cent bile) and pH tolerance (pH 2) than Saccharomyces cerevisiae. Prebiotic characterization results showed that the MOS (0.5 per cent and 1.5 per cent) extracted from RGT Saccharomyces cerevisiae significantly (P<0.01) improved Lactobacillus acidophilus growth. MOS derived from both Saccharomyces cerevisiae and RGT Saccharomyces cerevisiae, at all level of supplementation significantly (P<0.01) decreased the Escherichia coli growth.


Keywords: Saccharomyces cerevisiae, RGT Saccharomyces cerevisiae, Rabbit, Probiotic, Prebiotic MOS
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How to cite this article:

Elanthamil, R., C. Bandeswaran, P. Tensingh Gnanaraj and Vijayarani, K. 2018. Isolation and Characterization of Rabbit Gut Transmitted Saccharomyces cerevisiae as a Synbiotic.Int.J.Curr.Microbiol.App.Sci. 7(3): 871-882. doi: https://doi.org/10.20546/ijcmas.2018.703.102