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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 711-721
DOI: https://doi.org/10.20546/ijcmas.2018.703.083


Common Isolates among Suspected Cases of Septicemia with a Special Emphasis on Multidrug Resistant Strains
M. Shabnum*, P. Sreenivasulu Reddy and P. Vasundhara 
Department of Microbiology, Narayana Medical College, Nellore, Andhra Pradesh – 524003, India
*Corresponding author
Abstract:

To isolate common organisms from suspected cases of septicemia with their antimicrobial susceptibility pattern and know the prevalence of MDR strains by detecting MRSA isolates, ESBL, AmpC and Carbapenemase production. Blood samples were collected from suspected cases of septicemia and incubated in BacT/ALERT 3D. Subculture was done on Blood agar, nutrient Agar, MacConkey Agar and Chocolate Agar and identification was done by standard biochemical reactions. AST was done by Kirby Bauer Disc Diffusion method with MRSA, ESBL, AmpC and Carbapenemase producing isolates detected by using Cefoxitin, Ceftazidime and Ceftazidime + Clavulanic acid, Cefoxitin and Ceftriaxone and Modified Hodge Test respectively. Out of 300 samples, 24.66% were culture positive with Gram Negative Organisms being predominant [Klebsiella pneumoniae most common (29.72%) followed by E. coli] with highest resistance to Cefixime (91.67%), Cefoperazone + Sulbactam (83.33%). Coagulase Negative Staphylococcus (5.40%) was most common Gram Positive organism isolated which showed highest resistance to Ampicillin and Cefixime (75%). 18 were MDR strains with 16.21% ESBL producers, 6.71% Carbapenemase producers, 2.7% MRSA isolates. No AmpC producers were detected. Gram Negative Septicemia was more predominant with highest resistance to cefixime and sensitivity to Polymyxin B and Imipenem. 24.32% of total isolates were MDR strains. Continued Surveillance studies are needed for implementation of control measures to limit spread of MDR strains.


Keywords: Septicemia, ICU, MDR strains, MRSA, ESBL, Carbapenemases
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How to cite this article:

Shabnum, M., P. Sreenivasulu Reddy and Vasundhara, P. 2018. Common Isolates among Suspected Cases of Septicemia with a Special Emphasis on Multidrug Resistant Strains.Int.J.Curr.Microbiol.App.Sci. 7(3): 711-721. doi: https://doi.org/10.20546/ijcmas.2018.703.083