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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:3, March, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 441-447
DOI: https://doi.org/10.20546/ijcmas.2018.703.052


Isolation and Characterization of Nitrogen Fixing Bacteria from Babchi (Psorolea corylifolia L.) and Testing them for Plant Growth Promotion Traits in vitro
Mahesh Dattatry Shengepallu, Ravindra Tatyaram Gaikwad, Visvas Anandrao Chavan and Yumlembam Rupert Anand*
Department of Plant Pathology and Agricultural Microbiology, Mahatma Phule Krishi Vidyapeeth, Rahuri – 413 722 (M.S.), India
*Corresponding author
Abstract:

Two isolates of Rhizobium were isolated from Babchi (Psorolea corylifolia) root nodules, which showed positive tests for catalase test, oxidase test, nitrate reduction test and starch hydrolysis while negative test for methyl red test, Voges-Proskauer test, urease test, gelatin lignification test, methylene blue test, urease test, gelatin liquification test, methylene blue test, lactose test, citrate utilization test and hydrogen sulphide production test. The isolate PCRI-1 and PCRI-2 exhibited a great variation in N fixation by of 24.35 mg/g and 20.70 mg/g of sucrose consumed, respectively. The amount of IAA produced by these isolates was 37.73 µg/ml and 32.40 µg/ml respectively. These isolates showed variation in PO4 solubilization efficiency in the range of 19.1 to 17.8 per cent, with solubilization zone of 6.2 mm and 4.2 mm, respectively. Its characterization and testing of plant growth promotion traits is the first attempt from the state of Maharashtra, from root nodules of Babchi.


Keywords: Babchi (Psorolea corylifolia L.), in vitor

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How to cite this article:

Mahesh Dattatry Shengepallu, Ravindra Tatyaram Gaikwad, Visvas Anandrao Chavan and Yumlembam Rupert Anand. 2018. Isolation and Characterization of Nitrogen Fixing Bacteria from Babchi (Psorolea corylifolia L.) and Testing them for Plant Growth Promotion Traits in vitro.Int.J.Curr.Microbiol.App.Sci. 7(3): 441-447. doi: https://doi.org/10.20546/ijcmas.2018.703.052
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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