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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 416-425
DOI: https://doi.org/10.20546/ijcmas.2018.703.049


Development of an Indirect Enzyme-Linked Immunosorbent Assay Using Recombinant Microneme 10 (MIC10) Protein of Toxoplasma gondii for Diagnostic Evaluation in Murine Model Infected with Tachyzoites
Raghubir Singh1*, M.A. Malik1, M. Sankar2, Maninder Singh1, H.K. Sharma1 and K. Sahil3
1Division of Veterinary Public Health and Epidemiology, F.V.Sc & A.H, R.S. Pura, SKUAST-J, Jammu, India
2Temperate Animal Husbandry, Indian Veterinary Research Institute, Mukteshwar, Nainital, India
3Division of Livestock Production and Management, F.V.Sc & A.H, R.S.Pura, SKUAST-J, Jammu, India
*Corresponding author
Abstract:

Toxoplasma gondi being microscopic size and obligate intracellular, its proliferative forms cultivation in the laboratory is relative difficult and thus, immunodiagnostic tools such as serology and immune-histochemistry development becomes essential to demonstrate the infection in vivo. Among the various existing serological methods, the enzyme-linked immunosorbent assay (ELISA) is simple, rapid and economical, rendering it suitable for clinical diagnosis applications. The aim of present study was to express the microneme 10 (MIC10) antigenic protein which is a circulating antigen following invasion by tachyzoites in hosts in the prokaryotic expression system and evaluated as a potential diagnostic marker by indirect ELISA using the infected and non infected sera samples from Swiss albino mice. Optimum working dilutions of the antigen, sera and the conjugate were determined by checker board titrations and the cut off value was generated as mean plus standard deviation of optical densities of negative sera by ELISA. Recombinant MIC10-ELISA at the cut off value of 1.2539 showed the sensitivity and specificity as 79.2% and 87.2% respectively. The data was generated as a step to evaluate the potential of MIC10 antigen protein as a diagnostic marker.


Keywords: Toxoplasma, MIC10, ELISA, Sensitivity, Specificity
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How to cite this article:

Raghubir Singh, M.A. Malik, M. Sankar, Maninder Singh, H.K. Sharma and Sahil, K. 2018. Development of an Indirect Enzyme-Linked Immunosorbent Assay Using Recombinant Microneme 10 (MIC10) Protein of Toxoplasma gondii for Diagnostic Evaluation in Murine Model Infected with Tachyzoites.Int.J.Curr.Microbiol.App.Sci. 7(3): 416-425. doi: https://doi.org/10.20546/ijcmas.2018.703.049