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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 346-354
DOI: https://doi.org/10.20546/ijcmas.2018.703.040


Molecular Characterization of β-Tubulin Isotype-1 Gene of Trichostrongylus colubriformis
D.B. Bhinsara1, M. Sankar1*, V. Sudan3, B.J. Thakre4, R. Kumar1, A. Kumar2, B.C. Parthasarathi2, Bhawana Kushwaha1, Ravi Kumar Khare1, S. Shanmuganathan1 and D. Chandra2
1Indian Veterinary Research Institute, Mukteswar campus, Nainital, Uttrakhand - 243 186, India
2Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh-243 122, India
3Department of Parasitology, DUVASU, Mathura, Uttar Pradesh – 281001, India
4Department of Veterinary Parasitology, College of Veterinary Science and Animal Husbandry, JAU, Junagadh, Gujarat - 362 001, India
*Corresponding author
Abstract:

Benzimidazole resistance (BZR) is known to be associated with single nucleotide polymorphisms (SNPs) on beta-tubulin isotype 1 gene at any of F200Y, F167Y and E198A positions. Characterization of beta-tubulin isotype 1 gene is prerequisite for designing molecular diagnostic method for ascertaining BZR at the early stage of development. Present study was designed to characterize beta tubulin isotype-1 gene of Trichostrongylus colubriformis of small ruminants. Total RNA was extracted from male T. colubriformis. The β-tubulin gene complementary DNA (cDNA) was reverse transcribed from total RNA. The truncated β-tubulin gene of 1202 bp was PCR amplified from complementary DNA (cDNA), cloned in pGEM®-T easy TA cloning vector, custom sequenced and analysed. The analysis of deduced amino acid sequence revealed that the Uttarakhand isolate is susceptible to benzimidazole resistance as the amino acid on 200th position is phenylalanine (F200Y). The amino acid sequences showed 98.3% to 99% identity with the published T. colubriformis sequence as well as other trichstrongyle species. The phylogenetic analysis revealed that Uttarakhand isolate clustered with other trichostrongyles species. The information of beta tubulin isotype-1 gene of T. colubriformis provided an idea for development of molecular tools to diagnosis of benzimidazole resistance at the early stage.


Keywords: Benzimidazole resistance, Beta-tubulin isotype-1, Mutation, Trichostrongylus colubriformis
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How to cite this article:

Bhinsara, D.B., M. Sankar, V. Sudan, B.J. Thakre, R. Kumar, A. Kumar, B.C. Parthasarathi, Bhawana Kushwaha, Ravi Kumar Khare, S. Shanmuganathan and Chandra, D. 2018. Molecular Characterization of β-Tubulin Isotype-1 Gene of Trichostrongylus colubriformis.Int.J.Curr.Microbiol.App.Sci. 7(3): 346-354. doi: https://doi.org/10.20546/ijcmas.2018.703.040