Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 212-217
DOI: https://doi.org/10.20546/ijcmas.2018.703.025


Detection of Carbapenamase Resistance in Non-Fermenter Gram Negative Bacilli by Phenotypic Methods in A Tertiary Care Hospital
R.P.R. Suyambu Meenakshi*, S. Gowri, G. Sucilathangam and C. Revathy
Department of Microbiology, Tirunelveli Medical College, Tirunelveli - 627 011, Tamil Nadu, India
*Corresponding author
Abstract:

Non fermenter Gram negative bacilli were considered as contaminants or commensals of little significance1. However, recent literature review shows that these organisms are now associated with life-threatening infections such as Septicemia, Pneumonia, Urinary Tract Infection, Meningitis, Surgical Site Infection, Ventilator Associated Pneumonia, Wound Infection, Osteomyelitis etc. Carbapenemases are diverse enzymes that vary in their ability to hydrolyze Carbapenems and other beta lactams. Detection of Carbapenemase is a crucial infection control issue because they are often associated with extensive antibiotic resistance, treatment failures and infection‑ associated mortality. A total of 100 isolates of Non Fermenter Gram Negative Bacilli were isolated from specimens of Respiratory Tract Infection, Wound Infection, Urinary Tract Infections, Septicemia, Post Operative Wound Infections, Ear Infections. The isolated NFGNB were subjected to Meropenem resistance by disc diffusion test and those strains which show resistance to Meropenem were subjected to Carbapenamase detection method by using, Double disc synergy test, combination disc method and MBL E test. Among the 100 Non fermenter isolates, 87 were Pseudomonas aeruginosa and 13 were Acinetobacter baumanii. Screening with Meropenem disk showed 32% of isolates were positive for Carbapenem resistance. Among them CDT detected 59.3%, DDST 50% and E test 46.8% of the MBL producer. 13 MDR NFGNB were detected among the 32 isolates. The proper identification of NFGNB up to the species level together with monitoring their susceptibility patterns are mandatory for proper management of infections caused by these pathogens.


Keywords: Non fermenter Gram negative bacilli, Meropenem, Carbapenemase
Download this article as Download

How to cite this article:

Suyambu Meenakshi, R.P.R., S. Gowri, G. Sucilathangam and Revathy, C. 2018. Detection of Carbapenamase Resistance in Non-Fermenter Gram Negative Bacilli by Phenotypic Methods in A Tertiary Care Hospital.Int.J.Curr.Microbiol.App.Sci. 7(3): 212-217. doi: https://doi.org/10.20546/ijcmas.2018.703.025