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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 3657-3669
DOI: https://doi.org/10.20546/ijcmas.2018.702.435


Antifungal Activity of Palmatine against Strains of Candida spp. Resistant to Azoles in Planktonic Cells and Biofilm
Rosana de Sousa Campos1,2,7, Cecília Rocha da Silva1,2,7, João Batista de Andrade Neto1,2, Letícia Serpa Sampaio1,2, Francisca Bruna Stefany Aires do Nascimento1,2, Manoel O. de Moraes3, Bruno Coêlho Cavalcanti3, Hemerson Iury Ferreira Magalhães4, Akenaton Onassis Cardoso Viana Gomes5, Marina Duarte Pinto Lobo6 and Hélio Vitoriano Nobre Júnior1,2*
1Dept. of Clinical and Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection and Experiments in Yeast (LABEL), Federal University of Ceará, Fortaleza, CE, Brazil 2Department of Pathology and Legal Medicine, School of Medicine, Federal University of Ceará, Fortaleza, CE, Brazil 3Department of Physiology and Pharmacology, Neuropharmacology Laboratory, Federal University of Ceará, Fortaleza, CE, Brazil 4School of Pharmacy, Federal University of Paraíba, João Pessoa, PB, Brazil 5Department Organic and Inorganic Chemistry, Federal University of Ceará,Fortaleza, CE, Brazil 6School of Pharmacy, University of Fortaleza (UNIFOR), CE, Brazil 7Christus University Center (UNICHRISTUS), Fortaleza, CE, Brazil
*Corresponding author
Abstract:

The incidence of fungal infections has increased and it is necessary to seek new antifungals that also act against resistant strains. Palmatine is a protoberberine alkaloid noteworthy for its various pharmacological properties, including antifungal properties. The objective of this study was to evaluate the activity of palmatine against Candida spp. strains resistant to azole in planktonic cells and biofilm, and to elucidate the possible mechanism of cell death. Palmatine showed an antifungal activity with MICs ranging from 32 to 128 µg/mL. In the formed biofilms, palmatine reduced by 50% cell viability at the concentration of 20x MIC (C. albicans) and 10x MIC (C. tropicalis and C. parapsilosis). In the formed biofilms, the same percentage of inhibition was achieved at a concentration of 1x MIC (C. albicans) and 2x MIC (C. tropicalis and C. parapsilosis). It was found that palmatine causes damage to DNA, promotes mitochondrial depolarization and increasing the externalization of phosphatidylserine, suggesting cell death by apoptosis. The antifungal action of palmatine in Candida spp. resistant to azoles in planktonic cells and biofilm formed and in formation, as well as the fact of not having presented cytotoxicity in mammalian cells, point to a promising new anti-candida molecule.


Keywords: Candida spp., Palmatine, Biofilm, Flow cytometry, Comet assay
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How to cite this article:

Rosana de Sousa Campos, Cecília Rocha da Silva, João Batista de Andrade Neto, Letícia Serpa Sampaio, Francisca Bruna Stefany Aires do Nascimento, Manoel O. de Moraes, Bruno Coêlho Cavalcanti, Hemerson Iury Ferreira Magalhães, Akenaton Onassis Cardoso Viana Gomes, Marina Duarte Pinto Lobo and Hélio Vitoriano Nobre Júnior. 2018. Antifungal Activity of Palmatine against Strains of Candida spp. Resistant to Azoles in Planktonic Cells and Biofilm.Int.J.Curr.Microbiol.App.Sci. 7(2): 3657-3669. doi: https://doi.org/10.20546/ijcmas.2018.702.435