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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 3035-3051
DOI: https://doi.org/10.20546/ijcmas.2018.702.368


Characterization of Bt Transgenic Plants: A Review
Keshavareddy* and A.R.V. Kumar
Department of Entomology, University of Agricultural Sciences, GKVK,Bangalore-560065, Karnataka, India
*Corresponding author
Abstract:

Insecticidal δ-endotoxins of Bacillus thuringiensis have acquired great significance in recent years because of their specificity to target insects and toxicity at very low concentrations. Genes from B. thuringiensis have been the most successful group of genes identified for use in genetic transformation of crops for pest control on a commercial scale. Successes of plant transformation include most of major economic and vegetable crops. Polymerase chain reaction (PCR) is commonly used technique for the presence of transgene in the putative transformants by DNA amplification. The Southern blot technique is used to confirm gene integration and copy numbers and Northern blot for gene expression in transgenic plants. Quantification Bt protein expressed in plants is performed by Enzyme Linked Immunosorbent Assay (ELISA). Bt transgenic plants were assessed for insect resistance against target pest/s by conducting insect bio-assays.


Keywords: δ-endotoxins, Bt-transgenics plants, Quantification, Insect bio-assays
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How to cite this article:

Keshavareddy, G. and Kumar, A.R.V. 2018. Characterization of Bt Transgenic Plants: A Review.Int.J.Curr.Microbiol.App.Sci. 7(2): 3035-3051. doi: https://doi.org/10.20546/ijcmas.2018.702.368