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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 2846-2854
DOI: https://doi.org/10.20546/ijcmas.2018.702.347


Comparison of Total Phenolic Content, Total Flavonoid Content, Antioxidant capacity and Free Radical Scavenging Activity of Leaves of Elaeocarpus sphaericus and Roots of Pelargonium zonale
Ravi Deepika*, Jagtar Singh and Naindeep Kaur
Department of Biotechnology, Panjab University, Chandigarh, India
*Corresponding author
Abstract:

Elaeocarpus sphaericus is commonly known as Rudraksha belonging to family Elaeocarpaceae and Pelargonium zoanle is a perennial small shrub, belonging to family Geraniaceae are one of the oldest and popular medicinal plants, rich in antioxidants and phytonutrients. In this study, Methanolic leaf extract of E. sphaericus and root extract P. zoanle were prepared for the comparative determination of total phenolic content, total flavonoid content, total antioxidant capacity and free radical scavenging activity by using Folin-Ciocalteau method, aluminium chloride colorimetric method, phosphomolybdenum method, DPPH and ABTS assay respectively. According to results, TPC and TFC was higher in leaf extract of E. sphaericus with the value of 37.5mg GAE/g of DW(dry weight) and 53.3mg QE/g of DW of extract as compare to P.zoanle (TPC: 24mg GAE/g of DW and TFC: 21.05 QE/g of DW of extract). TAC of root extract of P.zoanle (25mg AAE/g of DW) was higher than leaf extract of E. sphaericus (14.28mg AAE/g of DW). In DPPH assay, % inhibition was higher in E. sphaericus with IC50 value of 477.125µg/ml in comparison to P. zoanle with IC50 value of 659.89µg/ml. ABTS assay showed that the free radical scavenging activity of both E. sphaericus (IC50 value = 2.96µg/ml) and P. zoanle (IC50 value = 2.96µg/ml) was lower than the synthetic antioxidant BHT (IC50 value = 12.56µg/ml). It was observed that both medicinal plants can be used in pharmaceutical and food industry in order to replace synthetic antioxidants with natural antioxidants.


Keywords: Elaeocarpus sphaericus, Pelargonium zoanle, Antioxidants, Total phenolic content (TPC), Total flavonoid content (TFC), Total antioxidant capacity (TAC) and free radicals
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How to cite this article:

Ravi Deepika, Jagtar Singh and Naindeep Kaur. 2018. Comparison of Total Phenolic Content, Total Flavonoid Content, Antioxidant capacity and Free Radical Scavenging Activity of Leaves of Elaeocarpus sphaericus and Roots of Pelargonium zonale.Int.J.Curr.Microbiol.App.Sci. 7(2): 2846-2854. doi: https://doi.org/10.20546/ijcmas.2018.702.347