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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 2767-2770
DOI: https://doi.org/10.20546/ijcmas.2018.702.337


Breed Related Hospital Prevalence of Theileria annulata infection in Cattle-calves Confirmed by Nested Polymerase Chain Reaction in Bikaner District of Rajasthan, India
Pavan Goyal* and Anju Chahar
Department of Epidemiology and Preventive Veterinary Medicine, College of Veterinary and Animal Science, Rajasthan University of Veterinary and Animal Sciences, Bikaner-334 001, Rajasthan, India
*Corresponding author
Abstract:

One hundred cattle-calves, out of which 37 cross bred and 63 indigenous, were screened for Theileria annulata infection during October-2015 to September-2016 brought to Teaching Veterinary Clinical Complex, College of Veterinary and Animal Science, Bikaner. Blood samples were collected in ethylene diamine tetra acetic acid (EDTA @1mg/ml) vacutainers from all the suspected cattle-calves for genomic DNA isolation and subjected for confirmatory diagnosis by nested polymerase chain reaction (nPCR). By nPCR, 19 cross bred and 22 indigenous cattle-calves were found positive for Theileria annulata infection. Thus, the prevalence of Theileria annulata infection in cross bred and indigenous cattle-calves was 51.35% (19/37) and 34.92% (22/63), respectively.


Keywords: Theileria annulata, Cattle-calves, DNA, nPCR, Cross bred, Prevalence
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How to cite this article:

Pavan Goyal and Anju Chahar. 2018. Breed Related Hospital Prevalence of Theileria annulata infection in Cattle-calves Confirmed by Nested Polymerase Chain Reaction in Bikaner District of Rajasthan, India.Int.J.Curr.Microbiol.App.Sci. 7(2): 2767-2770. doi: https://doi.org/10.20546/ijcmas.2018.702.337