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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 2119-2124
DOI: https://doi.org/10.20546/ijcmas.2018.702.253


Prokaryotic Expression and Confirmation of Non-structural Protein C of Peste-Des-Petits-Ruminants Virus
S. Bhadouriya1, D. Choudhary1, A. Yadav1, S. Chandra Sekar1, V.V. Dhanesh1, M. Sankar1, K.K. Rajak2, R.P. Singh2, M.A. Ramakrishnan1, A.B. Pandey2 and D. Muthuchelvan1*
1Division of Virology, ICAR-Indian Veterinary Research Institute, Mukteswar, Nainital District, Uttarakhand 263 138, India
*Corresponding author
Abstract:

Peste-des-petits-ruminants (PPR) is a transboundary viral disease of small ruminants. In the present study, the full length C gene was amplified from PPR vaccine virus (Sungri/96) and expressed in a prokaryotic expression system. Specificity of the recombinant C protein was confirmed through immune-blot using anti-His conjugate and hyperimmune sera from goats. The immunogenicity of the protein was assessed using monospecific sera raised in goats. The recombinant C protein produced in this study will be useful for the development of DIVA enabled vaccine and diagnostics.


Keywords: Peste-des-petits-ruminants, PPR, PPRV, Non-structural protein C, Prokaryotic expression
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How to cite this article:

Bhadouriya, S., D. Choudhary, A. Yadav, S. Chandra Sekar, V.V. Dhanesh, M. Sankar, K.K. Rajak, R.P. Singh, M.A. Ramakrishnan, A.B. Pandey and Muthuchelvan, D. 2018. Prokaryotic Expression and Confirmation of Non-structural Protein C of Peste-Des-Petits-Ruminants Virus.Int.J.Curr.Microbiol.App.Sci. 7(2): 2119-2124. doi: https://doi.org/10.20546/ijcmas.2018.702.253