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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 2085-2093
DOI: https://doi.org/10.20546/ijcmas.2018.702.248


Antagonistic Potential and Molecular Characterization of Trichoderma spp. against Rhizoctonia solani Infecting Ghost Pepper in Manipur, India
Khedarani Koijam and Bireswar Sinha*
Department of Plant Pathology, Central Agricultural University, Iroisemba-795004, India
*Corresponding author
Abstract:

The present study was undertaken to analyze the effect of the native Trichoderma spp., isolated from the rhizosphere of ghost pepper (Capsicum chinense) against root rot caused by Rhizoctonia solani. Rhizospheric Trichoderma spp. were isolated from ghost pepper growing sites of Manipur and screened for their biocontrol potential against Rhizoctonia solani. Four fungal isolates were isolated and selected based on morphology and growth pattern. Molecular identification was carried out and an amplicon size of 262 bp were generated for fungus. Screening of the potent isolates showed variation on the radial growth of the pathogen, Rhizoctonia solani. Seven different substrates viz. Rice bran, Rice bran + Sugarcane juice (10%), Rice bran+Cowdung (1:1)+Jaggery (3%), Rice bran+ Cowdung (1:1)+Sugarcane juice (10%), Rice bran+Sugar (3%), Rice bran+ Mustard cake (1:1) + Jaggery (3%), Rice bran+ Mustard cake (1:1) + Sugarcane juice (10%) were evaluated for mass production of the most potent Trichoderma spp. Among the different substrate, highest population was recorded in rice bran + mustard cake (1:1) + sugarcane juice (10%) from CAU (13.33 x 108cfug-1) isolate at 30 days of incubation.


Keywords: Biocontrol, Mass production, Screening
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How to cite this article:

Khedarani Koijam and Bireswar Sinha. 2018. Antagonistic Potential and Molecular Characterization of Trichoderma spp. against Rhizoctonia solani Infecting Ghost Pepper in Manipur, India.Int.J.Curr.Microbiol.App.Sci. 7(2): 2085-2093. doi: https://doi.org/10.20546/ijcmas.2018.702.248