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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(7): 289-294
DOI: http://dx.doi.org/10.20546/ijcmas.2016.507.030


A Comparative Analysis of Enzyme-Linked Immunosorbent Assay and Rapid Card Test for Diagnosis of Rotavirus Antigen in Acute Diarrhea Below Five Years Children
Jugal Kishor Agarwal1*, S.P. Garg1, Dayachand2 and Dipty Agrawal3  
1Department of Microbiology, SIMS, Hapur, India
2Department of Pediatrics, SIMS, Hapur, India
 3Department of Anesthesia, SDN Hospital, Delhi, India
*Corresponding author
Abstract:

Group-A Rotavirus are responsible for severe watery diarrhea in young children. Rapid diagnosis of Rotavirus associated diarrhea can prevent inappropriate administration of antibiotics and help in preventing the spread of multi- drug resistance.  Rotavirus antigen is detected by ELISA and with one step rapid antigen test showed an incidence of 22% using ELISA and 21.33% using Rapid test respectively. Rapid test showed a sensitivity of 96.97% and specificity of 100% in comparison to ELISA. The Rotavirus infection was highest in age group of 6 months to 24 months (62.77%) and in male (63.64%). The infection was maximum during winter and presented with triad of diarrhea, vomiting and fever. Majority of cases had watery diarrhea with severe dehydration.


Keywords: ELISA,Group A Rotavirus, Immuno-chromatography, Watery diarrhea.
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How to cite this article:

Jugal Kishor Agarwal, S.P. Garg, Dayachand and Dipty Agrawal. 2016.  A Comparative Analysis of Enzyme-Linked Immunosorbent Assay and Rapid Card Test for Diagnosis of Rotavirus Antigen in Acute Diarrhea Below Five Years ChildrenInt.J.Curr.Microbiol.App.Sci. 5(7): 289-294. doi: http://dx.doi.org/10.20546/ijcmas.2016.507.030