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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 1577-1586
DOI: https://doi.org/10.20546/ijcmas.2018.702.190


Morphological Changes in Fouquieria splendens Callus Cocultivated with Endophyte Bacteria
Daniel Corona-Álvarez1, Verónica Azeret Salinas-Patiño1, MaríaVictoria Hernández- Pimentel1, Silvano Montes-Villafan1, Marcial García-Pineda2, Leonor Angélica Guerrero-Zúñiga3 and Angélica Rodríguez-Dorantes1*
1Laboratorio de Fisiología Vegetal, Departamento de Botánica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México City 11340, México
2Jardín Botánico, Facultad de Estudios Superiores, Iztacala, Universidad Autónoma de México, Los Reyes Iztacala s/n, México City54090, México
3Instituto Mexicano del Petróleo, México City 07730, México
*Corresponding author
Abstract:

Functional associations between phytobacteria and cultured plants have often been successful; some authors suggest that co-cultivation of bacterial strains with in vitro cells or tissues, seems to be one of the appropriate approaches for mass propagation of plants. This study analyzed the effect on growth and development of a desert plant Fouquieria splendens callus, co-cultivated with an endophyte bacterium. From all the obtained results, F. splendens callus evaluated at 19 and 45 days showed no visible damage, discoloration and diminish of growth comparing between co-cultivated and no co-cultivated conditions. Particularly the plant regulators added to the medium and the presence of the endophyte bacteria, favored the increase in callus biomass of this plant species. Scanning electron microscopy analysis of the selected callus showed morphological changes induced in co-cultivated conditions with the development of an extracellular matrix, giving an early shoots development, as a differentiation event. In this work, the establishment of co-cultives between F. splendens callus and the streaked endophyte on culture medium showed that it could be a complex network of responses between the plant growth regulators present in culture medium and the release of phytohormones produced by the phytobacteria.


Keywords: Biotization, Callus culture, Endophytes, Co-cultive
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How to cite this article:

Daniel Corona-Álvarez, Verónica Azeret Salinas-Patiño, MaríaVictoria Hernández- Pimentel, Silvano Montes-Villafan, Marcial García-Pineda, Leonor Angélica Guerrero-Zúñiga and Angélica Rodríguez-Dorantes. 2018. Morphological Changes in Fouquieria splendens Callus Cocultivated with Endophyte Bacteria.Int.J.Curr.Microbiol.App.Sci. 7(2): 1577-1586. doi: https://doi.org/10.20546/ijcmas.2018.702.190