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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 988-997
DOI: https://doi.org/10.20546/ijcmas.2018.702.122


Specific Antiproliferative Activity against Several Human Cancer Cells Possessed by Cordyceps militaris Grown in Viable Pupa of Silkworm Raised under Sterile Environment
Yukiko Ogawa1*, Hiroko Murakami2, Takuya Kuraoka3, Ryukichi Mine2 and Hidemitsu Kobayashi3
1Divisions of Infection Control and Prevention, Department of Pharmacy, Faculty of Pharmaceutical Science, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki 859-3298, Japan
2Minejumokuen Co., Ltd., Nonoshima, Goshi, Kumamoto, 861-1103, Japan
3Divisions of Microbiology, Department of Pharmacy, Faculty of Pharmaceutical Science, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo,
Nagasaki 859-3298, Japan
*Corresponding author
Abstract:

We succeeded in large volume artificial cultivation of Cordyceps militaris, using silkworm live pupa obtained by aseptic breeding. The appearance (shape, color, and size) of fruiting bodies of cultured bacteria was almost the same as that of wild one. We investigated the antiproliferative inhibitory effect on eleven human cancer cells in vitro using the water extracted fraction obtained from this fruiting body homogenate. As a result, this soluble fraction effectively inhibited the growth of some cancer cells in a concentration-dependent manner. In particular, the effect on leukemia U937 cells was remarkable, but there was almost no effect on bile duct adenocarcinoma TYBDC-1 cells or pancreatic KP-3L cancer cells. In addition, almost no effect was exerted on three normal cells (human hepatocyte (NHH), human mammary epithelial cells (NHME), human epidermal melanocytes (NHEM)) at the same concentration. Therefore, it was revealed that the human cell proliferation inhibitory effect of the C. militaris water extract fraction greatly differs in susceptibility depending on the type of cancer cell under the concentration range not affecting normal cells. Furthermore, the anti-cancer effect of C. militaris was associated with an induction of cell cycle arrest and caspase-mediated apoptosis. This fact suggests that some compounds contained in metabolic fluids of these mushrooms can be lead compounds leading to the development of anticancer drugs with very few side effects.


Keywords: Cordyceps militaris, Silkworm, Bombyx mori, Anticancer activity, Medicinal plants
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How to cite this article:

Yukiko Ogawa, Hiroko Murakami, Takuya Kuraoka, Ryukichi Mine and Hidemitsu Kobayashi. 2018. Specific Antiproliferative Activity against Several Human Cancer Cells Possessed by Cordyceps militaris Grown in Viable Pupa of Silkworm Raised under Sterile Environment.Int.J.Curr.Microbiol.App.Sci. 7(2): 988-997. doi: https://doi.org/10.20546/ijcmas.2018.702.122