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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(7): 164-170
DOI: http://dx.doi.org/10.20546/ijcmas.2016.507.016


Purification and Characterization of a Novel CGTase from Alkaliphilic Bacillus flexus Isolated from Lonar lake, India
A.Shinde Vinod* and S.M. More
 Department of Microbiology, Yeshwant Mahavidyalaya, Nanded- 431602 (M. S.), India
*Corresponding author
Abstract:

Production and purification of Cyclodextrin glycosyltransferase (CGTase) from Alkaliphilic Bacillus flexus isolated from Lonar lake, India was investigated in present study. Production was carried out using medium containing starch, yeast extract, peptone MgSO4.7H2O. The crude enzyme was collected by centrifugation and partially purified using ammonium sulphate precipitation method. This partially purified enzyme was further purified using phenyl sepharose column chromatography .The enzyme obtained had molecular weight of 77.58 kDa which is confirmed by SDS PAGE and Mass spectroscopy.


Keywords: Cyclodextrin glycosyltransferase (CGTase); purification,Phenyl sepharose
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How to cite this article:

Shinde Vinod, A., and More, S.M. 2016. Purification and Characterization of a Novel CGTase from Alkaliphilic Bacillus flexus Isolated from Lonar lake, India.Int.J.Curr.Microbiol.App.Sci. 5(7): 164-170. doi: http://dx.doi.org/10.20546/ijcmas.2016.507.016