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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 3072-3080
DOI: https://doi.org/10.20546/ijcmas.2018.701.364


In vitro Regeneration of Recalcitrant Green Gram (Vigna radiata L. Wilczek) from Immature Cotyledons for Genetic Improvement
Aparna Priyadarshini Patra1*, Kailash Chandra Samal1, Gyana Ranjan Rout1,Simachal Sahu2 and Prem Narayan Jagadev2
1Department of Agriculture Biotechnology, College of Agriculture, Orissa University of Agriculture and Technology, Bhubaneswar, Odisha-751003, India
2Department of Plant Breeding and Genetics, College of Agriculture, Orissa University of Agriculture and Technology, Bhubaneswar, Odisha-751003, India
*Corresponding author
Abstract:

In the present investigation, a simple, rapid and efficient in vitro regeneration system was developed in green gram (Vigna radiata L. Wilczek) cv. IPM-02-03 using cotyledonary explants excised from a 4 day old seedling. Organogenic calli were developed from cotyledonary tissues within 4-6 weeks of culture, supplemented with 2.5 mg/L BAP and 1.5 mg/L NAA. Shoot regeneration was achieved from shoot tips and cotyledonary node on MS medium was supplemented with BAP 2.0 mg/l. Regeneration frequency depends on genotype, explants types and growth regulator combinations in the medium. 1.5 mg/l BAP in combination with 1.0 mg/l Kinetin was proved to be the most effective treatment for multiple shoots induction from nodal explants after 3-4 weeks. The number of shoots per culture varied from 1.3 to 7.8 in different growth media. The cultures were incubated for 16 hrs of photoperiod in 25oC for 2 weeks for shoot bud regeneration. The proliferated shoots were further sub cultured on similar medium for higher shoot bud regeneration. Rooting was achieved in all rooting media but elongated shoots were rooted on ½ strength MS media supplemented with 0.5 mg/l NAA or IBA with 2% (W/V) sucrose which produced the maximum number of strong and healthy roots. The rooted plantlets were transferred to soil mixture (Soil: Sand: Vermicompost 1:1:1 ratio) and kept in the green house with 85% humidity. Seventy-five percent plants were successfully grown after regeneration with no phenotypic variation. This protocol can be used for genetic transformation and large-scale production of genetically improved green gram variety.


Keywords: In vitro regeneration, Vigna radiata, BAP, NAA, IBA
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How to cite this article:

Aparna Priyadarshini Patra, Kailash Chandra Samal, Gyana Ranjan Rout, Simachal Sahu and Prem Narayan Jagadev. 2018. In vitro Regeneration of Recalcitrant Green Gram (Vigna radiata L. Wilczek) from Immature Cotyledons for Genetic Improvement.Int.J.Curr.Microbiol.App.Sci. 7(1): 3072-3080. doi: https://doi.org/10.20546/ijcmas.2018.701.364