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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 3048-3059
DOI: https://doi.org/10.20546/ijcmas.2018.701.362


Isolation, Identification and Antibiogram of Coagulase Negative Staphylococcus (CoNS) Isolated from Various Clinical Samples at a Tertiary Care Teaching Hospital, Jaipur, India
Shiv Kumar1, Jitendra3*, Anup Das2, Pratibha Mane4, Jyoti Sangwan4 and Saroj Kumari5
1Department of Microbiology, RNT Govt. Medical College, Udaipur, Rajasthan, India
2Department of Microbiology, NIMS Medical College, Jaipur, Rajasthan, India
3Department of Microbiology, 4Department of Microbiology,
5Department of Paediatrics, SHKM Govt. Medical College, Nalhar (Nuh), Haryana, India
*Corresponding author
Abstract:

Coagulase-Negative Staphylococcus (CoNS) comprise an ever-expanding group of bacteria whose medical importance has emerged in the past decades. They have become one of the most frequent nosocomial pathogens isolated from blood cultures, often in association with intravascular devices and as a cause of deep-seated prosthetic implant infections (Parameswaran et al., 2011). These organisms isolated from clinical specimens with increasing frequency. So this study planned to isolate and antibiogram of Coagulase Negative Staphylococcus (CoNS) bacteria in various clinical specimens at tertiary care teaching hospital at Jaipur. The present study was carried out on total 60 Coagulase Negative Staphylococcus (CoNS) were isolated from 400 different clinical samples like Urine, Blood, Pus, Sputum, High Vaginal Swab, Semen, Nasal Swab and ET Secretion etc. from all age’s group. Conventional bacteriological methods were used for identification Coagulase Negative Staphylococcus (CoNS) and susceptibility testing was performed with the help of the Modified Kirby-Bauer disc diffusion method as per CLSI guidelines 2014. This study comprises of 60 isolates of Coagulase Negative Staphylococcus (CoNS) from total of 400 clinical specimens collected from in patients admitted to various departments during a period of 6 months. Staphylococcus epidermidis was the most common isolate (38.33%) while Staphylococcus saprophyticus (35%) was the second most common isolated followed by Staphylococcus haemolyticus (15%), Staphylococcus lugdanensis (5%), Staphylococcus schleiferi and Staphylococcus xylosis have same account (3.33%). Among all clinical samples Urine yield maximum isolates of CoNS i.e. 26 (43.33%) followed by Blood 10 (16.67%), Pus 9 (15%), Sputum 6 (10%), High Vaginal Swab 5 (8.33%) etc. Most sensitive drug against CoNS were Vancomycin (100%) followed by Linezolid (86.67%), Amikacin (71.67%). The most common species identified was Staphylococcus epidermidis. Resistance to Penicillin and Amoxyclav was high and none of the isolates showed resistance to Vancomycin. Early detection of MRCoNS and reduction in indiscriminate use of antibiotics like Vancomycin are the only suitable approaches to reduce the emergence of these drug resistant strains.


Keywords: Coagulase Negative Staphylococcus (CoNS), MRCoNS, MSCoNS, Typical opportunist, Nosocomial infection, Deep seated prosthetic implant infection, Antimicrobial susceptibility, Multiple drug resistance
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How to cite this article:

Shiv Kumar, Jitendra, Anup Das, Pratibha Mane, Jyoti Sangwan and Saroj Kumari. 2018. Isolation, Identification and Antibiogram of Coagulase Negative Staphylococcus (CoNS) Isolated from Various Clinical Samples at a Tertiary Care Teaching Hospital, Jaipur, India.Int.J.Curr.Microbiol.App.Sci. 7(1): 3048-3059. doi: https://doi.org/10.20546/ijcmas.2018.701.362