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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 2768-2781
DOI: https://doi.org/10.20546/ijcmas.2018.701.332


Standardization of in vitro Culture Establishment and Proliferation of Micro-Shoots in African and French Marigold Genotypes
Ravindra Kumar1*, Kanwar Pal Singh2, D.V.S. Raju3, Sapna Panwar2,Reeta Bhatia4, Surendra Kumar2 and Pavanesh Kumar Verma2
1Dr.YSRHU, HRS-Kovvur, West Godavari Dist, Andhra Pradesh, India
2ICAR-Indian Agricultural Research Institute, New Delhi, India
3ICAR-Directorate of Floriculture Research, Pune, India
4ICAR-IARI Regional Station, Katrain, Himachal Pradesh, India
*Corresponding author
Abstract:

Marigold is native to Mexico and one of the commercial loose flower crops in India. In general it is commonly propagated through seeds, but some ornamentally high valued petaloid and gynomonoecious lines can only be maintained through vegetative propagation. Initial in vitro axenic culture establishment, poor multiplication rates, excess callusing and vitrified cultures are the major hindrances in its commercial micro-propagation. Therefore, the objective of the present investigation was to develop efficient in vitro protocol for mass multiplication of commercially popular African and French marigold cultivars Pusa Basanti Gainda (PBG) and Pusa Arpita (PA) respectively. Nodal segments were chosen as explant of these two open field cultivars. Explants were pre-treated with carbendazim (0.2%) + metalaxyl (0.2%) + 8-hydroxy quinoline citrate (200 mg/l) for 60 minutes followed by surface sterilization with 0.1% HgCl2 for 4 minutes to eliminate the microbial contamination. Highest culture establishment (69.44%) and earliest bud emergence (4.45 days) was recorded in Murashige and Skoog (MS) medium supplemented with BAP (2.0 mg/l) and NAA (0.05 mg/l). Among the different proliferation treatments, 100% proliferation was recorded in MS medium devoid of any growth regulators, MS + 0.5 mg/l Kinetin + 0.1 mg/l NAA and 0.5 mg/l BAP + 0.1 mg/l NAA + 2.5 mg/l AgNO3 supplemented media. The maximum numbers of quality shoots (4.3, 18.8, 64.2 and 208.2 shoots/explant) were obtained on MS medium supplemented with 0.5 mg/l BAP + 0.1 mg/l NAA + 2.5 mg/l AgNO3 in 30, 60, 90 and 120 days after culture respectively. This protocol is highly useful for mass multiplication of true-to-type, disease free planting material as well as helpful in long term maintenance of germplasm lines.


Keywords: African marigold, French marigold, Nodal segment, Micropropagation, Culture establishment, Vitrification, Proliferation
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How to cite this article:

Ravindra Kumar, K., Kanwar Pal Singh, D.V.S. Raju, Sapna Panwar, Reeta Bhatia, Surendra Kumar and Pavanesh Kumar Verma. 2018. Standardization of in vitro Culture Establishment and Proliferation of Micro-Shoots in African and French Marigold Genotypes.Int.J.Curr.Microbiol.App.Sci. 7(1): 2768-2781. doi: https://doi.org/10.20546/ijcmas.2018.701.332