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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 2740-2744
DOI: https://doi.org/10.20546/ijcmas.2018.701.328


Isolation and Identification of M. tuberculosis from Sheep Tissue Samples and Sero-Diagnosis Study in an Organized Sheep Farm
Arunmozhivarman1, R. Radhika1, P. Kannan2, V. Maroudam1,Vijayalakshmi1, P. Valentina Claudet1 and G. Dhinakar Raj1*
1Translational Research Platform for Veterinary Biologicals, Tamil Nadu Veterinary and Animal Sciences University, Chennai -600 051, India
2Department of Immunology, National Institute for Research in Tuberculosis,Chennai-31, India
*Corresponding author
Abstract:

A 2 years old female Madras Red sheep with the medical history of reduction in feed intake, poor weight gain and emaciation was found dead in an organised farm. The sheep did not have any obvious respiratory symptoms. Edematous and caseous lesions were observed in mesenteric, bronchial, mediastinal and prescapular lymph nodes of the sheep during post mortem examination. Other internal organs were free of any specific lesions. The lymph node samples were decontaminated and cultured by inoculating into BACTEC Mycobacteria Growth Indicator Tube (MGIT) system and Lowenstein Jensen slants. The cultures turned positive and acid fast staining of the bacterial culturerevealed the presence of Mycobacteria. The bacteria was further confirmed as Mycobacterium tuberculosis by multiplex PCR and nucleotide sequencing. A Tuberculosis sero-diagnostic study was conducted for all the animals in the farm using commercially available ELISA kit to know the incidence of tuberculosis in the farm. Three sheep out of the total 205 sheep were positive for tuberculosis by ELISA with the estimated 1.5% positivity. This shows the active circulation of tuberculosis in sheep farm and there may be possibility of human to animal transmission and vice versa. The role of sheep in the epidemiology and transmission of tuberculosis needs further study.


Keywords: Sheep, M. tuberculosis, PCR, ELISA, Seroprevalence
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How to cite this article:

Arunmozhivarman, K., R. Radhika, P. Kannan, V. Maroudam, K. Vijayalakshmi, P. Valentina Claudet and Dhinakar Raj, G. 2018. Isolation and Identification of M. tuberculosis from Sheep Tissue Samples and Sero-Diagnosis Study in an Organized Sheep Farm.Int.J.Curr.Microbiol.App.Sci. 7(1): 2740-2744. doi: https://doi.org/10.20546/ijcmas.2018.701.328