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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(6): 1026-1032
DOI: http://dx.doi.org/10.20546/ijcmas.2016.506.109


Molecular Identification of Pseudomonas aeruginosa from food borne isolates
D.B.M. Virupakshaiah* and V.B. Hemalata
Department of Biotechnology Basaveshwar Engineering College, Bagalkot, India
*Corresponding author
Abstract:

A research was done for the isolation and identification of undesirable microorganisms in food products using molecular approach the result revealed the presence of Pseudomonas aeruginosa in various food products by means of miss handling during harvesting and storage. Many of these isolates were resistant or reduced susceptibilities to multiple antimicrobial agents.The resistant bacteria isolates were selected for 16S rRNA  identification of Pseudomonas aeruginosa. 16S rRNA is a significant target to the molecular level identification of Pseudomonas aeruginosa. The results obtained were found to be a novel food borne pathogens, which were further named Pseudomonas aeruginosa strain HV17 and Pseudomonas aeruginosa strain HV77, after characterization the sequence of isolate was deposited in GenBank with accession numbers ‘KU982961’ and ‘KU982962’ respectively. 16S rRNA is the fundamental molecular method to identify organism Pseudomonas aeruginosa at strain level. Identified strain HV17 and HV77 and sequences will help for further research in the field of food borne pathogens.


Keywords: Pseudomonas aeruginosa, Resistant, Antimicrobial, Significant, Target
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How to cite this article:

Virupakshaiah, D.B.M., and Hemalata, V.B. 2016. Molecular Identification of Pseudomonas aeruginosa from food borne isolates.Int.J.Curr.Microbiol.App.Sci. 5(6): 1026-1032. doi: http://dx.doi.org/10.20546/ijcmas.2016.506.109