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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 2654-2659
DOI: https://doi.org/10.20546/ijcmas.2018.701.317


Comparison of the Diagnostic Efficacy of Rapid Immunochromatographic Test with Microwell ELISA in the detection of Dengue NS1 Antigen - A Retrospective Study
Krithikaa*, Marquess Raj, Silambarasi, Prabhu and Pramila
Department of Microbiology, Apollo Diagnostics, Regional Reference Laboratory (Apollo Health & Lifestyle Ltd.), No:6, 9th Avenue, Rathan Building, Ashok Nagar, Chennai-600083
*Corresponding author
Abstract:

Dengue fever is a Flavirus disease transmitted by Aedes mosquito. Primary dengue virus infection is characterized by elevations in specific NS1 antigen levels 0 to 9 days after the onset of symptoms. This retrospective study was conducted to assess performance of rapid ICTs in acute dengue diagnosis in comparison to ELISA test. It was conducted on 2234 serum samples received at The Department of Microbiology, Apollo Diagnostics, Regional Reference Laboratory, Chennai from September 2017 to November 2017 by ICT and ELISA to detect NS1 antigen. Total NS1 positive samples were 763(34.1%) by Rapid ICT and 815(36.5%) by ELISA. Five hundred and thirteen (513-63%), 234(28.7%) and 214(26.3%) of the positive samples belonged to male, female and children respectively. Monthly analysis of the data during the study period showed that 318(39.2%), 383(46.9%) and 114(13.9%) of the dengue NS1 antigen were detected during the months of September, October and November 2017 respectively. The sensitivity of Dengue NS1 Rapid ICT when compared with ELISA was found to be 93.6% and specificity was found to be 100%. ELISA has a minimal edge over Rapid ICT in the detection of Dengue NS1 antigen. Hence ICT in combination with ELISA could help in early and accurate diagnosis of acute dengue infection.


Keywords: DENV, NS1 antigen, Rapid Immuno chromatographic Test (ICT), Enzyme Linked Immunosorbent Assay (ELISA), Sensitivity, Specificity
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How to cite this article:

Krithikaa, S., Marquess Raj, Silambarasi, Prabhu and Pramila. 2018. Comparison of the Diagnostic Efficacy of Rapid Immunochromatographic Test with Microwell ELISA in the Detection of Dengue NS1 Antigen- A Retrospective Study.Int.J.Curr.Microbiol.App.Sci. 7(1): 2654-2659. doi: https://doi.org/10.20546/ijcmas.2018.701.317