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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(6): 1001-1006
DOI: http://dx.doi.org/10.20546/ijcmas.2016.506.106


Detection of Biofilm Coding icaD Gene in Methicillin Resistant Staphylococcus aureus (MRSA) Isolated from Patients undergoing Tonsillectomy in Basra City
Abdulameer Abdullah Al-Mussawi*
College of Nursing, University of Basra, Basra, Iraq
*Corresponding author
Abstract:

The aim of the present study was to detect biofilm formation of Methicillin Resistant  Staphylococcus aureus (MRSA) by two methods. One hundred forty two bacterial isolates were obtained  from the core of (63) infected tonsils collected from Al-Basra General Hospital in Basra city, cultured on orientation chromagar.34 isolates were identified as Staphylococcus aureus, out of which 33 isolates were identified as MRSA by using CHROMagar MRSA. Biofilm formation of the 33 MRSA  isolates were screened by Congo red agar (CRA) and iced gene detection. Thirty three isolates out of 34 isolates (97%) were identified asS. aureus, and 33 isolates (100%) as MRSA on CHROMagar media. The females (19 patients) were more than males (14 patents) with percentages (57.6%,42.4%) respectively. Twenty patients aged from (1-9 years), nine patients aged from (10-19 years),three aged from (20-29 years),and one patient aged from (30-39 years) represented (58.8%, 29.4%, 8.8% and 3%) respectively. Fourteen  (42.4%) isolates showed a black crystalline morphology, indicating biofilm production was observed on Congo red agar and 33(100%) in molecular method by detection of icaD gene. The molecular methods gave the most accurate to determine the MRSA producing of biofilm compared routine methods.


Keywords: Biofilm, icaD gene, Methicillin Resistant, Staphylococcus aureus, MRSA, tonsillectomy, CHROMagar, Basra
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How to cite this article:

Abdulameer Abdullah Al-Mussawi. 2016. Detection of Biofilm Coding icaD Gene in Methicillin Resistant Staphylococcus aureus (MRSA) Isolated from Patients undergoing Tonsillectomy in Basra City.Int.J.Curr.Microbiol.App.Sci. 5(6): 1001-1006. doi: http://dx.doi.org/10.20546/ijcmas.2016.506.106