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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 2373-2380
DOI: https://doi.org/10.20546/ijcmas.2018.701.285


Bio-formulation of Halotolerant Phosphate Solubilizing Enterobacter cloacae HFZ-H4 Strain to Screen Different Carrier Materials and their Shelf Life Study
Mohammad Hafeez1, P. W. Ramteke1, Rubina Lawrence1, Rambharose2,B.G. Suresh3, Supriya Kumari1, Ajay Kumar Singh1, Ankit Singla4,Anupriya Paul5, Shalini Masih5 and Harison Masih1*
1Department of Industrial Microbiology, Jacob Institute of Biotechnology and Bioengineering (JSBB), Sam Higginbottom University of Agriculture, Technology and Sciences (SHUATS), Allahabad, Uttar Pradesh-211007, India
2Department of Soil and Environmental Sciences, 3Department of Genetics and Plant Breeding SHUATS, India
4Regional Centre of Organic Farming, Ministry of Agriculture and Farmers Welftare, Govt. of India, Bhubaneswar, 751021
5Department of Mathematics and Statistics, SHUATS, India
*Corresponding author
Abstract:

The present research work was conducted to screen different carrier materials for halotolerant phosphate solubilizing Enterobacter cloacae HFZ-H4 strain to be used as bio-fertilizer formulation and study of their shelf life. Formulations of Enterobacter cloacae HFZ-H4 strain in fly-ash, saw dust and rice husk ash were screened for the better survival as bacterial bio-fertilizers formulation and result showed that Fly ash was found as best carrier material for bio-formulation and fly ash was further used as carrier material for optimization and shelf life study stored at optimized condition up to six months. Optimization of Fly ash based bio-formulation revealed that 25oC temperature and 30% moisture content were find as best condition for bio-fertilizer formulation to survive. Shelf life study in (fly ash) revealed that the population of bacteria gradually decreases with storage time and maximum population was observed at zero day (51 x 108 cfu/g) of formulation. The population of Enterobacter cloacae HFZ-H4 was declined upto 06 log at 108cfu/g after six months of storage which is within the permissible limit. From results of this study it can be concluded that the halotolerant strain of Enterobacter cloacae HFZ-H4 formulations in fly ash could be a better option for the growth and yield of the crop in sodic/saline soil.


Keywords: Bio-formulatoin, Halotolerant, Enterobacter cloacae, Shelf life and Fly-ash
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How to cite this article:

Mohammad Hafeez, P.W. Ramteke, Rubina Lawrence, Rambharose, B.G. Suresh, Supriya Kumari, Ajay Kumar Singh, Ankit Singla, Anupriya Paul, Shalini Masih and Harison Masih. 2018. Bio-formulation of Halotolerant Phosphate Solubilizing Enterobacter cloacae Strain to Screen Different Carrier Materials and their Shelf Life Study.Int.J.Curr.Microbiol.App.Sci. 7(1): 2373-2380. doi: https://doi.org/10.20546/ijcmas.2018.701.285