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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 2348-2362
DOI: https://doi.org/10.20546/ijcmas.2018.701.283


Isolation of Lipase-Producing Yeasts from Industrial Oily Residues in Different Culture Media
Michele Putti Paludo1, Kelly Silva Degani de Oliveira2,Thalles Canton Trevisol2 and Janaina Fernandes de Medeiros Burkert2*
 
1Federal University of Rio Grande (FURG), Chemistry and Food School (EQA), Campus Carreiros: Avenida Itália km 8 Bairro Carreiros, Rio Grande, Brazil
2Federal University of Rio Grande (FURG), Chemistry and Food School (EQA),Rio Grande, Brazil
*Corresponding author
Abstract:

The main goal of this work was to collect industrial oily residues and isolate lipolytic yeasts by selecting the best strain in relation to lipolytic and esterification activities and investigate the increase of production in shake flasks. A total of 78 yeasts were isolated on Yeast Malt agar and 44 strains with lipase-producing potential pre-selected on tributyrin agar. The yeast identified as Yarrowia lipolytica showed the highest lipolytic and esterification activities, reaching 2.67 U/mL and 464.65 U/g, respectively, at 48 h. Medium 4 showed major lipolytic (2.23 U/mL) and esterification (324.98 U/g) activity, concomitantly. The components of this medium were used in the factorial design; it was observed that an increase in the concentration of MgSO4 and olive oil decreased lipolytic activity significantly (p<0.05), and the other variables did not influence lipase production. The esterification activity and cell concentration were not affected significantly (p>0.05) for any of the variables studied. An increase in lipolytic activity was reached in trial 9 (5.12 U/mL) and trial 1 (5.23 U/mL) and the maximum esterification activity was 133.19 U/g (trial 14). Additionally, the trial 15 showed relevant activities (4.32 U/mL and 105.22 U/g) beyond the cell concentration (10.30 g/L), obtained concomitantly.


Keywords: Esterification activity, Factorial design, Screening, Lipolytic yeast, Yarrowia lipolytica
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How to cite this article:

Michele Putti Paludo, Kelly Silva Degani de Oliveira, Thalles Canton Trevisol and Janaina Fernandes de Medeiros Burkert. 2018. Isolation of Lipase-Producing Yeasts from Industrial Oily Residues in Different Culture Media.Int.J.Curr.Microbiol.App.Sci. 7(1): 2348-2362. doi: https://doi.org/10.20546/ijcmas.2018.701.283