International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:6, June, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(6): 990-1000

Establishing Enzyme Inhibition and membrane stability studies in methanolic extracts of Evolvulus alsinoides
Raja Rao1, S. Muralidhar, Talkad2*, H.V. Anil Kumar3 and H.R. Umesh4
1Research Scholar, Kalinga University, Campus: VillageKotni, Near Mantralay, Naya Raipur, Pin-492101, India
2P.G. Department of Biotechnology, R&;D Centre, Dayananda Sagar University, Kumaraswamy Layout, Bangalore-560078, India 3Department of Environmental Science & Laboratory for Applied biological Sciences, DVS College of Arts and Science, Shimoga, Karnataka, India 4P.G. Department of Biochemistry, R&;D Centre, Dayananda Sagar Institutions, Kumaraswamy Layout, Bangalore-560078, India
*Corresponding author

Shankhpushpi is botanically termed as Evolvulus alsinoides; the extracts have exhibited antioxidant, anti-ulcer, and immunomodulatory activities. In addition, recently scientific research has disclosed adaptogenic and anti-amnesic properties. The main objective of the present work is to investigate the invitro therapeutic potential activities of methanolic extract of whole plant of Evolvulus alsinoides on albumin denaturation, membrane stabilization test and Proteinase, xanthine oxidase, lipoxygenase inhibitory activities Establishing Anti inflammatory properties, Inhibition of albumin denaturation showed Albumin denaturation in methanolic plant extract (500μg/ml) 82.32 ± 0.50 against standard Aspirin (100μg/ml) 68.40 ± 0.40. Membrane stabilization plant extracts:  65.70 ± 0.60 against standard Aspirin: 84.20±0.40.  Xanthine oxidase IC50 (𝜇M) ± SEM, methanolic extract: 0.42 ±1.40 against standard Allopurinol: 0.20 ± 0.40. IC50 of Anti-lipoxygenase Activity (500μg/ml): 52.60 against standard Indomethacin (50μg/ml): 46.50%. The extract fractions serve as free radical inhibitors or scavenger or acting possibly as primary oxidants and inhibited the heat induced albumin denaturation, proteinase, lipoxygenase and xanthine oxidase (XO), which are agents that directly inhibit the synthesis of uric acid in vivo. Certain active constituents present in Evolvulus alsinoides plant extracts like flavonoids and polyphenolic compounds possess potent Anti inflammatory therapeutic responses.

Keywords: Evolvulus alsinoides, methanolic extract, flavonoids, polyphenolic compounds, proteinase, lipoxygenase and xanthine oxidase inhibitions

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Raja Rao, S. Muralidhar, Talkad, H.V. Anil Kumar and Umesh, H.R. 2016. Establishing Enzyme Inhibition and membrane stability studies in methanolic extracts of Evolvulus alsinoides.Int.J.Curr.Microbiol.App.Sci. 5(6): 990-1000. doi:
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