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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 1776-1785
DOI: https://doi.org/10.20546/ijcmas.2018.701.215


Genetic Diversity Analysis for Salinity Tolerance in Indian Mustard [Brassica juncea (L.)] Using SSR Markers
Rekha Patel1*, Ram C. Yadav1, Ram Avtar2, Sumit Jangra1, Geeta D. Boken2, Baldeep Singh2, and Neelam R. Yadav1
1Department of Molecular Biology, Biotechnology and Bioinformatics
2Department of Genetics and Plant Breeding, CCS Haryana Agricultural University, Hisar-125004, Haryana, India
*Corresponding author
Abstract:

Genetic diversity was performed among F2 plants of the cross RH 30×CS 52 in Indian mustard (Brassica juncea) (CS 52 is salinity tolerant and RH 30 is salinity susceptible) using SSR markers. Out of 358 SSR markers, 42 were found polymorphic and 154 were monomorphic. A total of 225 alleles, ranging from 2 to 4 were amplified. The PIC (Polymorphic Information Content) value ranged from 0.427-0.730 of Jaccard’s similarity coefficients was generated between these F2 populations. F2 population was grouped in 2 major clusters and 37 sub-clusters at a similarity coefficient of 0.58. The allelic information indicated that the genotypes used in the present study have distinct genetic patterns. It was concluded that SSR markers are more reliable and will be helpful in selecting diverse genotypes which can be used to identify QTL related to salinity.


Keywords: Brassica juncea, Genetic diversity, SSR markers, Dendrogram and salinity tolerance
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How to cite this article:

Rekha Patel, Ram C. Yadav, Ram Avtar, Sumit Jangra, Geeta D. Boken, Baldeep Singh and Neelam R. Yadav. 2018. Genetic Diversity Analysis for Salinity Tolerance in Indian Mustard [Brassica juncea (L.)] Using SSR Markers.Int.J.Curr.Microbiol.App.Sci. 7(1): 1776-1785. doi: https://doi.org/10.20546/ijcmas.2018.701.215