International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:6, June, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(6): 941-948

Detection and Molecular Characterization of Listeria species in ‘Wara’, A West African Local Cheese Sold in Ekiti State
Oyinloye, Josiah Mofoluwaso Adedeji*
Afe Babalola University, Biological Sciences Department, PMB 5454,Ado Ekiti, Ekiti State, Nigeria
*Corresponding author

Bacterial analysis of 116 soft cheeses ‘wara’, a ready-to-eat (RTE) product of fresh milk, bought in major markets of Ekiti State was done. Molecular analysis of isolates was done by coupling PCR to the DNA sequencing of Listeria 16S rRNA gene. Results yielded 459 bacteria with 78% positive to Listeria. Six different species of Listeria were identified with L. monocytogenes occurring at 12.4% of total Listeria. Ampiclox (ampicillin/cloxacillin) and amoxicillin showed very weak potency against all isolates at 10% and 11% respectively; while ciprofloxacin and septrin (co-trimoxazole) showed high potency at 84.95 and 82.6% respectively. The status of the RTE soft cheese ‘wara’ as sold in these is non-hygienic, hence microbiologically unsafe for consumption.

Keywords: Cheese,Wara, RTE,L. monocytogenes

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How to cite this article:

Oyinloye, Josiah Mofoluwaso Adedeji. 2016. Detection and Molecular Characterization of Listeria species in ‘Wara’, A West African Local Cheese Sold in Ekiti State.Int.J.Curr.Microbiol.App.Sci. 5(6): 941-948. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.