International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:6, June, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(6): 916-924

Isolation and Screening of Bacillus Species from Sediments and Application in Bioremediation
A.L. Tariq1*, S. Sudha1 and A.L. Reyaz2 
1Department of Microbiology, Sree Amman Arts and Science College, Erode-638102, India

2Department of Biotechnology, Periyar University, Salem-636011, Tamil Nadu, India
*Corresponding author

In the present study Bacillus species were isolated and screened for bioremediation applications. The quantifiable analysis showed Bacillus flora in septclean 154xl05 CFU/ml in nutrient agar and 243x105 CFU/ml in nutrient agar containing glucose. In river sediment, the Bacillus flora in the nutrient agar was 59x105 CFU/ml and 104xI05 CFU/ml in nutrient agar containing glucose while the marine sediment in nutrient agar 48xl05 CFU/ml and 69xl05 CFU/ml in nutrient agar containing glucose. The isolates were found to be gram positive rod shaped spore forming motile bacteria. The biochemical analysis was prepared to recognize the qualitative potential by the following conventional tests like starch hydrolysis, casein hydrolysis, gelatin liquefaction, cellulose test, urease test, carbohydrate fermentation, oxidation fermentation, Indole test, Methyl red, Voges Proskauer, Citrate utilization, Triple Sugar Iron, Nitrate reduction, Catalase  and Oxidase test. Bacillus species were showed their richness in protease, amylase, gelatinase, catalase, cellulase and urease enzymes. Fermentation capability for different sugars like glucose, sucrose, lactose, maltose and mannitol was determined. The three species of Bacillus were predominant which include Bacillus subtilis, Bacillus polvmyxa and Bacillus megaterium in septclean samples while in sediment sample Bacillus subtilis was found to be the common inhabitant. The Bacillus isolates from septclean flora and sediments showed good potential in reducing coliforms from polluted aquarium water, dye reduction and oil degradation. Efficiency for dye reduction and growth in oil was also high. The Bacillus flora proved an excellent agent for bioremediation in aquarium water tanks and their application can be extended further in treating waste water effluent from industries and to the polluted environment.

Keywords: Bioremediation, Bacillus species, Dye reduction, Coliform reduction,Effluent treatment.

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How to cite this article:

Tariq, A.L., S. Sudha and Reyaz, A.L. 2016. Isolation and Screening of Bacillus Species from Sediments and Application in Bioremediation.Int.J.Curr.Microbiol.App.Sci. 5(6): 916-924. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.