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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 1451-1456
DOI: https://doi.org/10.20546/ijcmas.2018.701.176


Salmonella enterica serovar Miami Possessing Both Virulence and Extended-Spectrum β-Lactamase Resistant Genes Isolated from Diarrhoeic Piglets of North East India (Mizoram)
Hosterson Kylla*, Tapan Kumar Dutta, Parimal Roychoudhury and Prasant Kumar Subudhi
Department of Veterinary Microbiology, Central Agricultural University, Aizawl, Mizoram 796 014, India
*Corresponding author
Abstract:

The present study report findings of an acute gastroenteritis outbreak in an unorganized pig farm in the tribal area of North East India (Mizoram). Faecal samples were collected from 12 pigs including 2severely diarrhoeic piglets. A total of 25 E. coli were isolated, all of which were found to be negative for any putative virulence genes of STEC, ETEC, EHEC and EPEC pathotypes. However, no samples were found to be positive for Clostridium spp, viral (Rotavirus, Picobirna virus) and parasitic pathogens (eggs or larvae) under the study. Salmonella isolates (n=2) were isolated and confirmed as Salmonella enterica serovar Miami (9,12:a:1,5).PCR amplification of the genes in study showed present of enterotoxin (stn), invasive (invA) as well as ESBL blaTEM-1 and blaCMY-2 genes. Analysis of the strains by RAPD-PCR indicates that the strains were genetically different from other Salmonellae. The resistance trait from the isolates could not be transferred to the recipient host by the conjugation method. The isolated Salmonella strains were found to be multi drug resistant (MDR) strains. The present study report the isolation of Salmonella Miami strains possessing both virulence and ESBL genes, from severely diarrhoeic piglets in the tribal area of North East India (Mizoram).


Keywords: Piglets, Diarrhoea, Salmonella Miami, PCR, Virulence, Antimicrobial drug resistance
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How to cite this article:

Hosterson Kylla, Tapan Kumar Dutta, Parimal Roychoudhury and Prasant Kumar Subudhi. 2018. Salmonella enterica serovar Miami Possessing Both Virulence and Extended-Spectrum β-Lactamase Resistant Genes Isolated from Diarrhoeic Piglets of North East India (Mizoram).Int.J.Curr.Microbiol.App.Sci. 7(1): 1451-1456. doi: https://doi.org/10.20546/ijcmas.2018.701.176