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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(6): 898-907
DOI: http://dx.doi.org/10.20546/ijcmas.2016.506.097


Screening and Characterization of Cellulose Degrading Bacterial Isolates of Waste disposal Site
Vinay Mohan Pathak* and Ajeet Singh, Navneet
 Department of Botany & Microbiology, Gurukul Kangri University,Haridwar 249-404, Uttarakhand, India
*Corresponding author
Abstract:

Cellulose is an abundant natural biopolymer on earth and most dominating agricultural waste. Cellulosic biomass is a renewable and abundant energy source with great potential for bioconversion to value-added bio-products. Cellulose can be degraded by cellulase producing bacteria which is most important biocatalysts for the industrial process. Under favorable environmental conditions, such enzymes facilitate the degradation of cellulosic material and stored carbon in the form of carbon. These microorganisms are diverse in nature and occur in a different habitat. In present investigation isolates were investigated for cellulose degrading potential. Four isolates V8, C25, V1, V4 were Bacillus sp., Pseudomonas sp., Pseudomonas sp. and Acinetobacter sp. screened for cellulose degradation. Carboxymethyl cellulose solution was used to analyze the cellulose degradation. Each isolates were examined for cellulose degradation potential with endo β-1, 4 glucanase activity and exo-β-1, 4 glucanase activities. All four tested isolates were showed good positive for both endo and exo β-1, 4 glucanase activity and cellulose degradation potential with 33.33 %, 8.53%, 3.84%, 3.12% weight loss, by isolates V1, V8, V4, C 2 5 respectively.


Keywords: Cellulose, carboxymethyl cellulase,bacteria,enzymes
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How to cite this article:

Vinay Mohan Pathak and Ajeet Singh, Navneet. 2016. Screening and Characterization of Cellulose Degrading Bacterial Isolates of Waste disposal SiteInt.J.Curr.Microbiol.App.Sci. 5(6): 898-907. doi: http://dx.doi.org/10.20546/ijcmas.2016.506.097