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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 1103-1109
DOI: https://doi.org/10.20546/ijcmas.2018.701.133


Cold Tolerance at Germination and Seedling Stages of Rice: Methods of Evaluation and Characterization of Thirty Rice Genotypes under Stress Conditions
Javeed A. Lone1*, M. Nisar Khan2, M. Ashraf Bhat1, Asif B. Shikari3, Shabir H. Wani4, Najeeb R. Sofi4, M. Imran Khan5 and Rayees A. Lone1
1Division of Genetics and Plant Breeding, FoA, Sher-e- Kashmir University of Agricultural Science and Technology of Kashmir, Wadura Sopore-193201, Jammu and Kashmir, India
2Dryland Agriculture Research Station, Budgam, India
3Division of Plant Biotechnology, Sher-e- Kashmir University of Agricultural Science and Technology, Shalimar-190025, India
4Sher-e-Kashmir University of Agricultural Science and Technology Shalimar, Rice Research Station (MRCFC) Khudwani, India
5Division of Agri-Statistics, Sher-e- Kashmir University of Agricultural Science and Technology, Shalimar-190025, India
*Corresponding author
Abstract:

Rice is one of the most important staple foods among the cereal crops for half of the human population. Unlike other cereals such as wheat and barley, rice plants are susceptible to cold stress, which causes decrease in productivity, especially in regions where the indica subspecies is cultivated. Low temperatures can have direct impacts on rice plants during germination, vegetative growth, and reproductive stages. This study aims to identify a suitable technique to estimate rice (Oriza sativa L.) cold tolerance at the germination stage and identify the variability among 30 rice genotypes. Cold tolerance was evaluated by two methods: in method I, rice seeds were kept and germinated under two conditions; 28 days in 13°C and seven days in 28°C; in experiment II, seedlings were kept at 72 hours 96 hours at 13°C and again 72 hours at 28°C. In experiment I percentages of reduction in radicle and coleoptile length and germination percent and percent decline in germination were measured. In experiment II coleoptiles growth radical regrowth after the cold period were measured. Results showed that low temperature effects in both experiments were varied among studied genotypes. Highest germination was achieved in SKAU-402 fallowed by SI-6, K-332, SI-5 and SI-3 with less percent decline in germination that is, 0.13, 0.17, 0.24 and 0.32 at 13ºC. Percentage of coleoptiles growth by low temperature (13ºC) was varied from 13.8 to 86.6%. In experiment II, radicle regrowth values varied from 2.0 to 12.0 mm, and SI-6,-SI-5, Kamad, SKAU-402 and K332, were the genotypes with high radicle regrowth.


Keywords: Abiotic stress, Controlled situation, Coleoptile growth, Radical regrowth, Low temperature
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How to cite this article:

Javeed A. Lone, M. Nisar Khan, M. Ashraf Bhat, Asif B. Shikari, Shabir H. Wani, Najeeb R. Sofi, M. Imran Khan and Rayees A. Lone. 2018. Cold Tolerance at Germination and Seedling Stages of Rice: Methods of Evaluation and Characterization of Thirty Rice Genotypes under Stress Conditions.Int.J.Curr.Microbiol.App.Sci. 7(1): 1103-1109. doi: https://doi.org/10.20546/ijcmas.2018.701.133