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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(6): 784-789
DOI: http://dx.doi.org/10.20546/ijcmas.2016.506.087


Study of Uropathogenic Multidrug Resistant Escherichia coli and Klebsiella Species with Reference to Extended Spectrum Beta Lactamase and Amp C Beta Lactamase Detection by Phenotypic Method
Surender Kaur* and Abhijit Awari
Department of Microbiology, Peoples College of Medical Sciences and Research Centre, Bhopal-462037 (M.P), India
*Corresponding author
Abstract:

Urinary tract infections are one of the most common bacterial infections in humans, both in the community as well as in the hospital settings. This study shows the changing antibiotic resistance pattern is pertinent for an appropriate treatment, prevention and control of the different mechanisms of resistance. Aim of this study was Detection of ESBL and AmpC in multidrug resistant uropathogenic E.coli and Klebsiella spp. E.coli & Klebsiella spp. were isolated by conventional method from 115 urine samples received in department of microbiology from Oct.2012 to Sept. 2013. The  antimicrobial  susceptibility  to  various drugs  was  studied  by  the  disc  diffusion  method,  by  following the  CLSI  guidelines. Isolates were diagnosed  for ESBL and AmpC production by phenotypic method i.e. Phenotypic Confirmatory Test for ESBL and Modified Hodge test using Cefoxitin disk for AmpC production. Total 115 isolates were collected for the study (E.coli-87 & Klebsiella spp-28), of which 52(45.21%) isolates were resistant to third generation cephalosporin. Out of 52 isolates, 28(53.8%) were ESBL producers and 13(25%) were AmpC producers.08 (21.1%) isolates were negative by both tests. From our study we concluded that it is essential to report ESBL and AmpC β-lactamase production along with the routine susceptibility testing, which will help the clinicians in prescribing antibiotics.


Keywords: ESBL,Amp C,E.coli,PCT,MHT, UTI.
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How to cite this article:

Surender KaurÍ™ and Abhijit Awari. 2016. Study of Uropathogenic Multidrug Resistant Escherichia coli and Klebsiella Species with Reference to Extended Spectrum Beta Lactamase and Amp C Beta Lactamase Detection by PhInt.J.Curr.Microbiol.App.Sci. 5(6): 784-789. doi: http://dx.doi.org/10.20546/ijcmas.2016.506.087