International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:6, June, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(6): 718-730

Isolation and Qualitative Selection of Fungi for Production of Lignocellulolytic Enzymes
Ajit Kaur1* and Urmila Gupta Phutela2
1Department of Microbiology, College of Basic Sciences and Humanities, Punjab Agricultural University, Ludhiana-141004, Punjab, India
2School of Energy Studies for Agriculture, College of Agricultural Engineering and Technology, Punjab Agricultural University, Ludhiana-141004, Punjab, India
*Corresponding author

Microorganisms especially Fungi are well known for their capability to excrete industrially important enzymes into the environment. In this purposed study, thirty four mesophilic as well as thermophilic fungal species were isolated from different sources like, decaying wood, decaying kinnow fruit, compost and soil.  The isolated species were then purified and qualitatively selected for their cellulolytic, lignolytic and hemicellulose degrading potential using agar plate assay method. Carboxy Methyl Cellulose was used as substrate for cellulase activity,  xylan was used as a substrate for xylanase activity; Remazol brilliant blue (RBB) dye and guaiacol were used as substrate to evaluate lignolytic activity in the isolated fungi. A total of 15 fungal isolates and the standard cultures namely Phanerochaete chrysosporium MTCC  787, Trichoderma reesei MTCC 164, T. harzianum MTCC 792,  Coriolus versicolor MTCC 138,  Pleurotus ostreatus MTCC 142,  Aspergillus oryzae NCIM 1212,  Penicillium roquefortii NCIM 712, Thermoascus aurantiacus MTCC 375, Humicola fuscoatra MTCC 1409  showed redness zone on guaiacol. Two mesophilic isolates namely A4 and A5; two thermophilic isolates A25 and A31 are the potential cellulose, hemicelluloses and lignin degraders which can further be used for enhancing biogas production. Among standard cultures P. chrysosporium and T. aurantiacus produced remarkable amounts of lignocellulolytic enzymes.

Keywords: Lignocellulolytic enzymes, Mesophilic and Thermophilic fungi, Isolation, Screening.

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How to cite this article:

Ajit Kaur and Urmila Gupta Phutela. 2016. Isolation and Qualitative Selection of Fungi for Production of Lignocellulolytic EnzymesInt.J.Curr.Microbiol.App.Sci. 5(6): 718-730. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.