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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 4088-4101
DOI: https://doi.org/10.20546/ijcmas.2017.612.470


Isolation and Identification of Dermatophytes in a Tertiary Care Hospital
Chandana Konda*, J.K. Surekha, I. Jahnavi, D. Sudha Madhuri and K. Nagamani
Department of Microbiology, Gandhi hospital, Musheerabad, Secunderabad, Telangana 500003, India
*Corresponding author
Abstract:

There is an increased prevalence of dermatophytes over the past few decades. This study was done to isolate and identify dermatophytes in patients attending dermatology outpatient department in a tertiary care hospital. A total of 100 skin, hair and nail samples from clinically suspected cases of dermatophytosis of all age groups and of both sexes were collected and processed by KOH and culture on Sabouraud dextrose agar and Dermatophyte test media. Out of 100 clinically suspected cases of Dermatophytosis, commonly affected age group was 21-30 yrs. (M: F = 1.43:1). Culture positive cases were 40%. Sensitivity and Specificity for KOH and culture of all samples were 90% and 75% respectively. Distribution of clinical lesions based on site were Tinea corporis (31%), T. unguium (26%), T. cruris (25%), T. facei (6%), T. capitis (4%), T. pedis (4%), T. barbae (2%), T. manuum (2%). Frequency of various species of dermatophytes isolated by culture were Trichophyton tonsurans (42.5%), T. mentagrophytes (20%) T. verrucosum (20%), T. rubrum (7.5%), Epidermophyton floccosum (5 %), T. violaceum (2.5 %) and Microsporum gypseum (2.5%). To conclude, in this study, most common clinical type of dermatophytosis was Tinea corporis. Most common dermatophyte isolated from skin scrapings was T. tonsurans followed by T. mentagrophytes. Dermatophytes isolated from nail samples were T. verrucosum and T. tonsurans. Dermatophyte isolated from hair was T. violaceum.


Keywords: Dermatophytes, KOH, Culture, Sabouraud dextrose agar, Dermatophyte test medium.
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How to cite this article:

Chandana Konda, J.K. Surekha, I. Jahnavi, D. Sudha Madhuri and Nagamani, K. 2017. Isolation and Identification of Dermatophytes in a Tertiary Care Hospital.Int.J.Curr.Microbiol.App.Sci. 6(12): 4088-4101. doi: https://doi.org/10.20546/ijcmas.2017.612.470