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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(6): 699-706
DOI: http://dx.doi.org/10.20546/ijcmas.2016.506.076


A Comparative in vitro Propagation Studies on Different Explants of Salvadora oleoides Decne-An Endangered Plant
Surender Kumar1,2*, Jitender Singh Laura1 and Narender Singh3
1Department of Bio-Sciences (Environmental Science), M. D. University Rohtak, India
2Department of Botany, K.M. Govt. P.G. College Narwana, India
3Department of Botany, K. U. Kurukshetra, India
*Corresponding author
Abstract:

Salvadora oleoides Decne belonging to family Salvadoraceae was selected for present study. This species is declining very rapidly due to various man made adverse activities like indiscriminate collection, over exploitation and loss of habitat. Therefore, in order to conserve and propagate this species, all modern micropropagation strategies were employed for rapid multiplication of this species. In this reports biotechnological technique especially micropropagation showed significant results from propagation point of view.  Various explants like shoot tips, Nodal segments and cotyledonary nodes were cultured on Murashige and Skoog’s medium. There was a diverse response of different explants regarding concentration of different growth regulators. However, growth regulators used in combination supported better results in terms of per cent regeneration and number of shoots. The different explants obtained from in vitro grown seedlings, gave better results under higher concentration of growth regulators whereas explants obtained from field grown mature tree showed better results supplemented with lower concentration of growth regulators. Furthermore, explants obtained from in vitro grown seedlings bore out better in terms of per cent regeneration of shoots and number of shoot regenerated.  Rhizogenesis was achieved on full strength MS medium fortified with auxins. The present research strongly recommends the maximum use of micropropagation techniques for rapid multiplication of true to type plants of Salvadora oleoides Decne.


Keywords: In vitro, Propagation, Cytokinins, Auxins, Explants, Salvadora oleoides.
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How to cite this article:

Surender Kumar, Jitender Singh Laura and Narender Singh. 2016. A Comparative in vitro Propagation Studies on Different Explants of Salvadora oleoides Decne-An Endangered PlantInt.J.Curr.Microbiol.App.Sci. 5(6): 699-706. doi: http://dx.doi.org/10.20546/ijcmas.2016.506.076