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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 3970-3975
DOI: https://doi.org/10.20546/ijcmas.2017.612.457


Screening of Endophyte Piper betle Bacteria from the Forests of HPPB University Andalas as Antibiotics Producer
Anthoni Agustien1*, Putra Santoso1, Nespy Permata Sari1, Fathya Annisa1, Nasril Nasir1, Yetria Rilda2 and Akmal Djamaan3
1Department of Biology
2Department of Chemistry
3Faculty of Pharmacy, Andalas University, Kampus Limau, Padang, Indonesia
*Corresponding author
Abstract:

Today livelihood exploration of endophytic microorganisms as a new source of antibiotics is very intensive, especially from medicinal plants. Piper betle, a green betel plant, is a medicinal plant. So that bacterial isolates from Piper betle are likely to produce antibacterial compounds. This study aims to screen the endophytic bacteria of green betel plants that live wild in HPPB forest, Andalas University. The leaf sampling was done by purposive random sampling, bacterial isolation was done by spread plate method on tryptic soy agar (TSA). Screening of antibiotic-producing endophytic bacteria was done by paper dish method. The results showed that isolation of endophytic bacteria using TSA medium obtained 86 isolates. Screening of antibiotic-producing bacteria was obtained by eleven isolates with six isolates having antibiotic activity against E. coli and S. aureus test bacteria; one isolate was only active against E. coli alone and four isolates were only active against S. aureus. Endophytic bacterial, isolates BES-2 has the largest inhibitory zone diameter (18.58 mm) and includes strong category antibiotics.


Keywords: Screening, Endophytic bacteria, P. betle, Antibiotics, Inhibitory zone.
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How to cite this article:

Anthoni Agustien, Putra Santoso, Nespy Permata Sari, Fathya Annisa, Nasril Nasir, Yetria Rilda and Akmal Djamaan. 2017. Screening of Endophyte Piper betle Bacteria from the Forests of HPPB University Andalas as Antibiotics Producer.Int.J.Curr.Microbiol.App.Sci. 6(12): 3970-3975. doi: https://doi.org/10.20546/ijcmas.2017.612.457