International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:6, June, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(6): 674-678

Assessment of Genetic Diversity by Using RAPD Markers of Vigna unguiculata cultivars in Telangana State, India
A,Srujana* and N. Lakshmi Bhavani
 Plant Tissue Culture and Plant Molecular Genetics Lab, Department of Botany, University College of science, Saifabad, Osmania University, Hyderabad.500004, India
*Corresponding author

In the present study genetic diversity based on RAPD markers was carried for five high yielding cultivars of Vigna unguiculata from Telangana region. A total 16 primers were used for the detection of polymorphism. Out of 230 bands formed 109 bands were found to be polymorphic. However the extent of polymorphism varied with each primer present and an average of 91.1% polymorphism was found among the cultivars in Vigna. Dendrogram constructed based on UPGMA analysis showed two clusters with first cluster bifurcating into two subclusters a and b. Dendrogram and similarity index clearly indicated that Gangothri and Pusakomal are in the same cluster a, and two cultivars Navrathan and Gowthami in cluster b,of the first cluster representing two genetically similar groups.  Gomchi found to be genetically much distant and formed a second cluster indicating that this cultivar is much variant from others.

Keywords: Cowpea ,Vigna unguiculata,Genetic Diversity RAPD Markers,Dendrogram

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How to cite this article:

Srujana, A., and Lakshmi Bhavani, N. 2016. Assessment of Genetic Diversity by Using RAPD Markers of Vigna unguiculata cultivars in Telangana State, India.Int.J.Curr.Microbiol.App.Sci. 5(6): 674-678. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.