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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(6): 674-678
DOI: http://dx.doi.org/10.20546/ijcmas.2016.506.073


Assessment of Genetic Diversity by Using RAPD Markers of Vigna unguiculata cultivars in Telangana State, India
A,Srujana* and N. Lakshmi Bhavani
 Plant Tissue Culture and Plant Molecular Genetics Lab, Department of Botany, University College of science, Saifabad, Osmania University, Hyderabad.500004, India
*Corresponding author
Abstract:

In the present study genetic diversity based on RAPD markers was carried for five high yielding cultivars of Vigna unguiculata from Telangana region. A total 16 primers were used for the detection of polymorphism. Out of 230 bands formed 109 bands were found to be polymorphic. However the extent of polymorphism varied with each primer present and an average of 91.1% polymorphism was found among the cultivars in Vigna. Dendrogram constructed based on UPGMA analysis showed two clusters with first cluster bifurcating into two subclusters a and b. Dendrogram and similarity index clearly indicated that Gangothri and Pusakomal are in the same cluster a, and two cultivars Navrathan and Gowthami in cluster b,of the first cluster representing two genetically similar groups.  Gomchi found to be genetically much distant and formed a second cluster indicating that this cultivar is much variant from others.


Keywords: Cowpea ,Vigna unguiculata,Genetic Diversity RAPD Markers,Dendrogram
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How to cite this article:

Srujana, A., and Lakshmi Bhavani, N. 2016. Assessment of Genetic Diversity by Using RAPD Markers of Vigna unguiculata cultivars in Telangana State, India.Int.J.Curr.Microbiol.App.Sci. 5(6): 674-678. doi: http://dx.doi.org/10.20546/ijcmas.2016.506.073