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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(6): 655-664
DOI: http://dx.doi.org/10.20546/ijcmas.2016.506.071


Isolation and Identification of a Bacteriocinic Substance Producing Bacteria from Various Sources
S.B. Ranganath* and T. Sharmila
Department of Microbiology and Biotechnology, Jnanabharathi Campus,Bangalore University, Bangalore- 560056, India
*Corresponding author
Abstract:

Bacteriocins  are low molecular  proteinaceous antimicrobial compounds  secreted  by  bacteria  to  inhibit  the  growth  of similar  or  closely  related  bacterial  strains. In the present study, an attempt was made to isolate  bacteria from various sources and screen for bacteriocinic activity. A total of 609 bacteria were isolated from different sources out of which 303 gram positive bacteria were screened for antimicrobial activity by primary streak method. Among these, 51 positive isolates were studied for colony and cell morphology which exhibited good antimicrobial activity. Thirty two isolates were streamlined for determination of bacteriocinic activity by agar well diffusion assay against four pathogens. Further three isolates IB23, AH4 and BrMk4 revealed significantly better antimicrobial activity against S. aureus, P. aeruginosa and E. coli. very scarce antimicrobial activity was observed against L. monocytogenes. Based on the biochemical tests, the organism was identified to be Staphylococcus aureus, Bacillus cereus and Lactobaccilus sp. respectively.


Keywords: Bacteriocin,Gram positive, Antimicrobial activity,pathogens,isolates.
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How to cite this article:

Ranganath, S.B., and Sharmila, T. 2016. Isolation and Identification of a Bacteriocinic Substance Producing Bacteria from Various Sources.Int.J.Curr.Microbiol.App.Sci. 5(6): 655-664. doi: http://dx.doi.org/10.20546/ijcmas.2016.506.071