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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(6): 642-654
DOI: http://dx.doi.org/10.20546/ijcmas.2016.506.070


HPTLC Finger Print Profile and Evaluation of in vitro Antimicrobial Activity of Mirabilis jalapa L.  Flowers
K.Kalaivani and G. Prasanna*
PG and Research Department of Biochemistry, Sengamala Thayaar Educational Trust Womens College, Sundarakkottai, Mannargudi, Thiruvarur Dt, Tamil Nadu, India
*Corresponding author
Abstract:

The present study has been designed to carry out the phytochemical screening, HPTLC finger print profile and in vitro antimicrobial activity of aqueous flowers extract of Mirabilis jalapa. Preliminary phytochemical screening confirmed the presence of alkaloids, anthraquinones, glycosides, flavonoids, tannins, terpenes etc., In HPTLC analysis, quercetin bands were identified in sample chromatogram (5 and 10  μl) which was confirmed by the chromatogram obtained from the standard quercetin and by comparing retention factors of quercetin (Rf - 0.51) value is from sample and standard solution.  In vitro antimicrobial activity of plant extract ( 50, 100 and 150 µg/ml) was also performed by using disc diffusion method against the pathogenic bacteria such as Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, and E.coli, pathogenic fungi such as Candida albicans and Trichoderma viride.  150 µg of aqueous flowers extract was active against both the bacterial and fungal species. Standard antibiotics gentamicin, amphotericin-B were also performed for antibacterial and antifungal activity respectively. The results of our study indicated that flavonoid quercetin is present in the plant extract which may responsible for potent antimicrobial activity against pathogenic microorganisms.


Keywords: Antimicrobial, flowers extract, HPTLC,Mirabilis jalapa, phytochemical,quercetin.
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How to cite this article:

Kalaivani, K., and Prasanna, G. 2016. HPTLC Finger Print Profile and Evaluation of in vitro Antimicrobial Activity of Mirabilis jalapa L.  Flowers.Int.J.Curr.Microbiol.App.Sci. 5(6): 642-654. doi: http://dx.doi.org/10.20546/ijcmas.2016.506.070