International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:6, June, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(6): 606-611

Invitro Assay of Tolerance Limit of Microorganism Isolated from Methyl Parathion Amended Soil
L.B. Sujatha* and Boopathi Mahalaxmi
Department of zoology, Pachaiyappa’s college, Chennai-30, India
*Corresponding author

The present investigation was dealt with the study of tolerance limit of microorganism against various concentration of pesticide methyl parathion from the Indigofera field at Senji near Thindivanam and to isolate the plasmid from the tolerant strain responsible for degradation. The isolates were biochemically identified by standard method of Bergy’s Manual of Bacteriology. The microorganism could able to tolerate the highest concentration of Methyl parathion and grow up to 500µl/100ml of agar medium. The characterized microorganism was found to be Gram-Positive cocci Staphylococcus aureus which fermenting Mannitol salt agar and the plasmid of this strain was isolated by standard method. This investigation would be of great help to found the tolerant strains of microorganism from methyl parathion pesticides and also in future it could be genetically engineered to yield a highly resistant strain which would degrade methyl parathion on a large scale.

Keywords: Methyl Parathion, Pesticides, Soil microbes, Staphylococcus aureus.

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How to cite this article:

Sujatha, L.B.,  and Boopathi Mahalaxmi. 2016. Invitro Assay of Tolerance Limit of Microorganism Isolated from Methyl Parathion Amended Soil.Int.J.Curr.Microbiol.App.Sci. 5(6): 606-611. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.